Temporospatial Expression of Dmrt1 in Chicken Urogenital System (Gallus Gallus) using Whole Mount In Situ Hybridization

Temporospatial Expression of Dmrt1 in Chicken Urogenital System (Gallus Gallus) using Whole Mount In Situ Hybridization
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使用整体原位杂交研究 Dmrt1 在鸡泌尿生殖系统(内鸡)中的时空表达

DOI:
10.1556/abiol.64.2013.2.3
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发表时间:
2013
影响因子:
--
通讯作者:
Y. Gong
Y. Gong
中科院分区:
生物4区
文献类型:
--
作者:
Y. Yang;P. Gong;Yanping Feng;S. Li;X. Peng;Z. Ran;Y. Qian;Y. Gong

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Doublesex 和 mab-3 相关转录因子 1 (Dmrt1) 是一种 Z 连锁基因,推测决定鸟类性腺的表型。使用全原位杂交 (WISH) 在胚胎发生过程中的泌尿生殖系统中检查 Dmrt1 的性别差异表达。结果显示,Dmrt1在鸡性腺中呈现二态性表达,从第6.5天到第10.5天增加。 Dmrt1在雄性(ZZ)性腺中的表达量不是雌性(ZW)性腺中的两倍,表明除了Z染色体剂量效应外还存在其他调节机制。
Doublesex and mab-3 -related transcription factor 1 (Dmrt1) is a Z-linked gene that putatively determines the phenotype of gonads in birds. The sex differential expression of Dmrt1 was examined using wholemount in situ hybridization (WISH) in the urogenital systems during embryogenesis. The results revealed that Dmrt1 showed dimorphic expression in chicken gonads, which increased from day 6.5 to day 10.5. The expression of Dmrt1 in male (ZZ) gonads was not twice as much as in female (ZW) gonads, suggesting the existence of other regulatory mechanisms in addition to Z chromosome dosage effect.
DOI: 10.1073/pnas.0712244105
发表时间: 2008-02-19
影响因子: 11.1
作者:
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发表时间: 1990-07-19
期刊: NATURE
影响因子: 64.8
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通讯作者: GOODFELLOW, PN
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DOI: --
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期刊:
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