Growth and survival of Neoceratium hexacanthum and Neoceratium candelabrum under simulated nutrient-depleted conditions

Growth and survival of Neoceratium hexacanthum and Neoceratium candelabrum under simulated nutrient-depleted conditions
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模拟营养耗尽条件下 Neoceratium hexacanthum 和 Neoceratium candelabrum 的生长和存活

DOI:
10.1093/plankt/fbt098
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发表时间:
2014
影响因子:
2.1
通讯作者:
Aldridge D
Aldridge D
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
Aldridge D

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鞭毛藻在海水中很常见,它们的主要无机营养物质如氮和磷都已耗尽。利用培养分离物,我们研究了两种oceratiumum (N. hexacanthumandN.;)在低营养副热带环流(osg)的地表水中发现的低营养浓度下,烛台(candelabrum)可以光养生长。氮处理后,未见光养生长(表现为细胞数量变化、细胞分裂或细胞蛋白增加)。hexacanthumandN。烛台生长在低营养海水中。在单独的实验中,为了确定在低营养营养条件下的生存时间,68%的n。在北大西洋环流海水中停留1 ~ 10天后,六角形细胞能够恢复生长;11 ~ 20天后恢复的占40%,21 ~ 30天后恢复的仅占3%。单个细胞存活并继续分裂的最长时间是26天。这些发现表明,新生神经细胞可以在osg的地表水中存活50 - 30周,但为了维持其生长,必须定期从其他来源获得营养:通过短暂的富营养水上涌、吞噬和/或往返于营养线的运动。
The dinoflagellateNeoceratiumis commonly observed in oceanic waters, depleted in major inorganic nutrients such as nitrogen and phosphorus. Using culture isolates, we investigated whether twoNeoceratiumspecies (N. hexacanthumandN. candelabrum) can grow phototrophically at low nutrient concentrations found in surface waters of oligotrophic subtropical gyres (OSGs). No phototrophic growth (indicated by changes in cell numbers, the presence of dividing cells or cellular protein increase) was observed whenN. hexacanthumandN. candelabrumwere grown in low nutrient seawater. In separate experiments, to determine survival time under oligotrophic nutrient conditions, 68% ofN. hexacanthumcells were able to re-establish growth after spending 1–10 days in North Atlantic gyre seawater; 40% recovered after 11–20 days and only 3% recovered after 21–30 days. The longest period any single cell survived, and then went on to divide, was 26 days. These findings demonstrate thatNeoceratiumcells could remain viable for >3 weeks in surface waters of OSGs, but to sustain their growth nutrients must be obtained periodically from an alternative source: via ephemeral upwelling of nutrient-rich waters, phagotrophy and/or movement to and from the nutricline.
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