Transcript Profiling Analysis and ncRNAs' Identification of Male-Sterile Systems of Brassica campestris Reveal New Insights Into the Mechanism Underlying Anther and Pollen Development.
Transcript Profiling Analysis and ncRNAs' Identification of Male-Sterile Systems of Brassica campestris Reveal New Insights Into the Mechanism Underlying Anther and Pollen Development.
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甘蓝雄性不育系统的转录谱分析和 ncRNA 鉴定揭示了对花药和花粉发育机制的新见解
DOI：
10.3389/fpls.2022.806865
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发表时间：
2022
影响因子：
5.6
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Zhou D;Chen C;Jin Z;Chen J;Lin S;Lyu T;Liu D;Xiong X;Cao J;Huang L
雄性不育突变体是研究花药和花粉发育的重要材料。本研究对大白菜（Brassica campestris L.）SSP. chinensis Makino，syn. B。拉帕湾chinensis）、核雄性不育系（A系）、Polima细胞质雄性不育（CMS）系（P系）和Ogura CMS系（O系）沿着它们的保持系（B系）。共筛选出7,136个差异表达基因（DEG）、361个差异表达长链非编码RNA（lncRNA）（DEL）、56个差异表达microRNA（miRNAs）（DEMs）。根据不育系的败育特性，构建了与花药细胞分化、减数分裂胞质分裂、花粉壁形成和绒毡层发育相关的特异调控网络。在无孢子细胞P系中鉴定了与细胞分化相关的候选基因和lncRNA，其中16个与拟南芥spl/nzz突变体中的DEG共有。在减数分裂胞质分裂缺陷的A系中选择与细胞板形成有关的基因和lncRNA。此外，花粉壁形成和绒毡层发育基因在拟南芥中的直系同源物在三个不同的不育系中表现出不同的表达模式。在361个DEL中，预测35个与miRNA相互作用，包括28个靶标，47个内源性靶标模拟物和5个miRNA前体。进一步证明两种lncRNA分别是bra-miR 156和bra-miR 5718的功能前体。B中bra-miR 5718 HG的过表达。白菜型油菜使花粉管生长缓慢，导致花粉管变短，最终影响结实。我们的研究为芸苔属作物花粉发育过程中的分子调控特别是ncRNA相互作用提供了新的见解。
Male-sterile mutants are useful materials to study the anther and pollen development. Here, whole transcriptome sequencing was performed for inflorescences in three sterile lines of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis), the genic male-sterile line (A line), the Polima cytoplasmic male-sterile (CMS) line (P line), and the Ogura CMS line (O line) along with their maintainer line (B line). In total, 7,136 differentially expressed genes (DEGs), 361 differentially expressed long non-coding RNAs (lncRNAs) (DELs), 56 differentially expressed microRNAs (miRNAs) (DEMs) were selected out. Specific regulatory networks related to anther cell differentiation, meiosis cytokinesis, pollen wall formation, and tapetum development were constructed based on the abortion characteristics of male-sterile lines. Candidate genes and lncRNAs related to cell differentiation were identified in sporocyteless P line, sixteen of which were common to the DEGs in Arabidopsis spl/nzz mutant. Genes and lncRNAs concerning cell plate formation were selected in A line that is defected in meiosis cytokinesis. Also, the orthologs of pollen wall formation and tapetum development genes in Arabidopsis showed distinct expression patterns in the three different sterile lines. Among 361 DELs, 35 were predicted to interact with miRNAs, including 28 targets, 47 endogenous target mimics, and five precursors for miRNAs. Two lncRNAs were further proved to be functional precursors for bra-miR156 and bra-miR5718, respectively. Overexpression of bra-miR5718HG in B. campestris slowed down the growth of pollen tubes, caused shorter pollen tubes, and ultimately affected the seed set. Our study provides new insights into molecular regulation especially the ncRNA interaction during pollen development in Brassica crops.
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影响因子：
2.9
作者：
De Storme N;Geelen D
通讯作者：
Geelen D
影响因子：
30.5
作者：
Casero D;Sandoval S;Seet CS;Scholes J;Zhu Y;Ha VL;Luong A;Parekh C;Crooks GM
通讯作者：
Crooks GM
影响因子：
27.5
作者：
Chen, Liyuan;Bernhardt, Anne;Hellmann, Hanjo
通讯作者：
Hellmann, Hanjo
影响因子：
4.5
作者：
He J;Xu M;Willmann MR;McCormick K;Hu T;Yang L;Starker CG;Voytas DF;Meyers BC;Poethig RS
通讯作者：
Poethig RS
芜菁花粉发育和受精过程中长非编码RNA的系统鉴定
影响因子：
7.2
作者：
Huang, Li;Dong, Heng;Cao, Jiashu
通讯作者：
Cao, Jiashu