Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH.
Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH.
复制标题
DOI：
10.1083/jcb.200207115
复制
发表时间：
2002-12-09
期刊：
影响因子：
--
通讯作者：
Bickmore WA
中科院分区：
文献类型：
--
作者：
Mahy NL;Perry PE;Bickmore WA
基因可以在染色体区域内转录；然而，据报道，主要的组织相容性复合体基因座延伸到远离染色体区域的区域，并且这种情况的发生率与该区域的转录相关。 1 号染色体的表皮分化复合体区域也出现了类似的结果。这些数据表明，染色质从染色体区域表面解聚可能是由受协调调控的密集基因的转录引起和/或可能促进其转录。为了研究未协调调节的区域是否也会出现染色体区域可见范围之外的定位，我们检查了人类 11p15.5 的空间组织和小鼠 7 号染色体上的同线区域。该区域基因丰富，但其基因并未协调表达，而是在几种细胞类型中总体上发生高水平转录。我们发现 11p15.5 的染色质经常延伸远离 11 号染色体区域。还检测到高基因密度和高转录水平的其他区域的区域外定位。这被证明部分依赖于正在进行的转录。我们认为，影响细胞核中染色体组织的是局部基因密度和转录，而不是单个基因的活性。
Genes can be transcribed from within chromosome territories; however, the major histocompatibilty complex locus has been reported extending away from chromosome territories, and the incidence of this correlates with transcription from the region. A similar result has been seen for the epidermal differentiation complex region of chromosome 1. These data suggested that chromatin decondensation away from the surface of chromosome territories may result from, and/or may facilitate, transcription of densely packed genes subject to coordinate regulation. To investigate whether localization outside of the visible confines of chromosome territories can also occur for regions that are not coordinately regulated, we have examined the spatial organization of human 11p15.5 and the syntenic region on mouse chromosome 7. This region is gene rich but its genes are not coordinately expressed, rather overall high levels of transcription occur in several cell types. We found that chromatin from 11p15.5 frequently extends away from the chromosome 11 territory. Localization outside of territories was also detected for other regions of high gene density and high levels of transcription. This is shown to be partly dependent on ongoing transcription. We suggest that local gene density and transcription, rather than the activity of individual genes, influences the organization of chromosomes in the nucleus.
登录
查看更多内容
DOI：
10.1083/jcb.145.6.1119
发表时间：
1999-06-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Croft JA;Bridger JM;Boyle S;Perry P;Teague P;Bickmore WA
通讯作者：
Bickmore WA
影响因子：
4
作者：
Jeppesen, P
通讯作者：
Jeppesen, P
影响因子：
3.5
作者：
Engemann, S;Strödicke, M;Walter, J
通讯作者：
Walter, J
影响因子：
21.3
作者：
Brown, KE;Amoils, S;Fisher, AG
通讯作者：
Fisher, AG
DOI：
10.1073/pnas.95.25.14857
发表时间：
1998-12-08
影响因子：
11.1
作者：
Gabriel, JM;Higgins, MJ;Nicholls, RD
通讯作者：
Nicholls, RD