Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury.
Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury.
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酚类化合物白藜芦醇对缺血/再灌注损伤大鼠肾脏肾功能和共刺激粘附分子 CD86 表达的影响。
DOI：
10.1679/aohc.68.41
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发表时间：
2005
影响因子：
--
通讯作者：
T. Habuchi
中科院分区：
文献类型：
--
作者：
M. Saito*;S. Satoh;N. Kojima;H. Tada;Mitsuru Sato;Toshio Suzuki;H. Senoo;T. Habuchi
最近的研究表明，缺血/再灌注(I/R)损伤可增强血管内皮细胞表面共刺激黏附分子的表达。本研究探讨了酚类产物白藜芦醇对大鼠肾脏I/R损伤后肾功能及CD86表达的保护作用。将Wistar大鼠随机分为4组：1)I/R组，右肾切除，左肾蒂夹闭1小时；2)溶剂组，I/R+10%乙醇(0.1ml/kg/d)，1~7天；3)白藜芦醇组，I/R+白藜芦醇4 mg/kg/d；4)假手术组。分别于术前1天、术后1、3、7天(第0天)经尾静脉采血测定血清肌酐(Scr)水平。免疫荧光染色检测肾皮质CD86蛋白表达，实时逆转录聚合酶链式反应(RT-PCR)定量检测肾皮质CD86信使RNA(MRNA)表达。白藜芦醇组术后1、3天Scr水平明显低于I/R组和赋形剂组。免疫组织化学结果显示，白藜芦醇组肾小球内皮细胞和肾小管周围血管CD86的表达较I/R组或赋形剂组明显减弱。白藜芦醇组CD86基因表达水平明显低于缺血再灌注组和假手术组，约为假手术组的五分之一。我们的结果表明，白藜芦醇能显著减轻I/R损伤后的肾功能障碍，降低CD86的mRNA和蛋白表达。
Recent studies have suggested that an ischemia/reperfusion (I/R) injury enhances the expression of costimulatory adhesion molecules on the vascular endothelium. In the present study, we investigated the protective effects of resveratrol, a phenolic product, on the renal function and expression of CD86 in rat kidneys with I/R injury. Wistar rats were divided into four groups; 1) an I/R group with right nephrectomy and 1-hour clamping of the left renal pedicle; 2) a vehicle group, I/R plus 10% ethanol (0.1 ml/kg/day) administered by intra-peritoneal injection from day -1 through to 7; 3) a resveratrol group, I/R plus 4 mg/kg/day of resveratrol; and 4) a sham group. Blood samples were obtained via the tail vein at 1 day before, and 1, 3, and 7 days after the operation (day 0) for the measurement of serum creatinine (Scr) levels. The expression of CD86 protein was analyzed by immunofluorescence staining, and the level of CD86 messenger RNA (mRNA) was evaluated quantitatively by a real-time reverse transcription-polymerase chain reaction (RT-PCR) in the renal cortex at day 3. Scr levels of the resveratrol group were significantly lower than those of the I/R and vehicle groups on days 1 and 3 after the operation. From the immunohistochemical study, the expression of CD86 in the glomerular endothelium and peritubular vessels was found to be attenuated in the resveratrol group compared with the I/R or vehicle group. In the resveratrol group, the CD86 mRNA level was significantly lower than that in the I/R or vehicle group, and it was significantly decreased by about one fifth of that in the sham group. Our results suggest that resveratrol markedly reduces renal dysfunction and attenuates the mRNA and protein expression of CD86 following I/R injury.
影响因子：
56.9
作者：
FREEMAN, GJ;GRIBBEN, JG;NADLER, LM
通讯作者：
NADLER, LM
影响因子：
5.2
作者：
O. Mgbonyebi;J. Russo;I. Russo
通讯作者：
O. Mgbonyebi;J. Russo;I. Russo
B7 共刺激通路在实验性冷缺血/再灌注损伤中的作用。
DOI：
10.1172/jci119632
发表时间：
1997
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Takada,M;Chandraker,A;Nadeau,KC;Sayegh,MH;Tilney,NL
通讯作者：
Tilney,NL