Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin
Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin
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利用多孔树脂在填充床反应器中长期连续培养肝细胞
DOI：
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发表时间：
1994
影响因子：
3.8
通讯作者：
H. Fukuda
中科院分区：
文献类型：
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作者：
Hirotoshi Miyoshi;K. Yanagi;N. Ohshima;H. Fukuda
作为我们利用培养的肝细胞开发混合型人工肝支持系统的尝试的一部分，我们以网状聚乙烯醇缩甲醛(PVF)树脂为支持材料，研究了在填充床式反应器中孵育的肝细胞的长期代谢功能。使用填充床反应器(直径20 mm)进行长期(最长1周)灌流培养实验。分别在无血清和有血清条件下，分别负载5 0 0块PVF树脂(平均孔径2 5 0μm，2×2×2 mm)和常规单层培养实验。在反应动力学分析的基础上，对氨代谢和尿素合成活性进行了定量评价。以氨代谢和尿素氮合成的初始速率以及谷丙转氨酶活性作为衡量反应器代谢性能和培养肝细胞活性的指标。
As part of our attempt to develop a hybrid artificial liver support system using cultured hepatocytes, we investigated the long‐term metabolic function of hepatocytes incubated in a packed‐bed type reactor using reticulated polyvinyl formal (PVF) resin as a supporting material. Long‐term (up to 1 week) perfusion culture experiments using the packed‐bed reactor (20 mm i.d.) loaded with 500 PVF resin cubes (mean pore size 250 μm, 2 × 2 × 2 mm), together with conventional monolayer culture experiments as controls, were performed in serum‐free or serum‐containing medium. Ammonium metabolism and urea synthesis activities were evaluated quantitatively based on reaction kinetic analyses. Initial rates of ammonium metabolism and urea‐N synthesis, as well as GPT enzyme activities, were adopted as indexes of the metabolic performance of the reactor and activities of the cultured hepatocytes.
影响因子：
56.9
作者：
FRANGOS, JA;ESKIN, SG;IVES, CL
通讯作者：
IVES, CL