Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail.
Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail.
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DOI：
10.1371/journal.pone.0265008
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Ishishita S;Kitahara S;Takahashi M;Iwasaki S;Tatsumoto S;Hara I;Kaneko Y;Kinoshita K;Yamaguchi K;Harada A;Ohmori Y;Ohkawa Y;Go Y;Shigenobu S;Matsuda Y;Suzuki T
血红素的前体，原卟啉IX（PPIX），在鸟类的子宫中大量积累，如日本鹌鹑，日本鹌鹑，它有棕色斑点的蛋壳;然而，子宫中PPIX生产的分子基础仍然很大程度上未知。在这里，我们调查了在一个典型的日本鹌鹑突变体表现出白色蛋壳低PPIX生产的原因，通过比较其基因表达在子宫与野生型使用转录组分析。我们还进行了遗传连锁分析，以确定致病基因组区域的白色蛋壳表型。我们发现，11个基因，包括5 '-氨基乙酰丙酸合酶1（ALAS 1）和类肝素1（HEPHL 1），在野生型子宫特异性上调，在突变体下调。我们绘制了6号染色体上172 kb的候选基因组区域，该区域包含多个基因，包括编码转录因子的配对样同源结构域3（PITX 3）的一部分。ALAS 1、HEPHL 1和PITX 3在野生型鹌鹑的子宫腔上皮的顶端细胞和子宫粘膜的固有层细胞中表达，而它们的表达水平在突变鹌鹑的细胞中下调。使用子宫匀浆进行的生化分析表明，5 '-氨基乙酰丙酸的有限可用性是PPIX产量低的主要原因。这些结果表明，血红素生物合成相关基因的子宫特异性转录调控是日本鹌鹑蛋壳色素生产的进化获得机制。基于这些发现，我们讨论了PPIX在日本鹌鹑子宫生产的分子基础。
The precursor of heme, protoporphyrin IX (PPIX), accumulates abundantly in the uteri of birds, such as Japanese quail, Coturnix japonica, which has brown-speckled eggshells; however, the molecular basis of PPIX production in the uterus remains largely unknown. Here, we investigated the cause of low PPIX production in a classical Japanese quail mutant exhibiting white eggshells by comparing its gene expression in the uterus with that of the wild type using transcriptome analysis. We also performed genetic linkage analysis to identify the causative genomic region of the white eggshell phenotype. We found that 11 genes, including 5’-aminolevulinate synthase 1 (ALAS1) and hephaestin-like 1 (HEPHL1), were specifically upregulated in the wild-type uterus and downregulated in the mutant. We mapped the 172 kb candidate genomic region on chromosome 6, which contains several genes, including a part of the paired-like homeodomain 3 (PITX3), which encodes a transcription factor. ALAS1, HEPHL1, and PITX3 were expressed in the apical cells of the luminal epithelium and lamina propria cells of the uterine mucosa of the wild-type quail, while their expression levels were downregulated in the cells of the mutant quail. Biochemical analysis using uterine homogenates indicated that the restricted availability of 5’-aminolevulinic acid is the main cause of low PPIX production. These results suggest that uterus-specific transcriptional regulation of heme-biosynthesis-related genes is an evolutionarily acquired mechanism of eggshell pigment production in Japanese quail. Based on these findings, we discussed the molecular basis of PPIX production in the uteri of Japanese quails.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
7.7
作者：
Chung J;Nakatsu F;Baskin JM;De Camilli P
通讯作者：
De Camilli P
影响因子：
3.9
作者：
Heinemann, Ilka U.;Jahn, Martina;Jahn, Dieter
通讯作者：
Jahn, Dieter
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
3.7
作者：
Li, Zhuanjian;Ren, Tuanhui;Kang, Xiangtao
通讯作者：
Kang, Xiangtao