Tachykinin-3 Genes and Peptides Characterized in a Basal Teleost, the European Eel: Evolutionary Perspective and Pituitary Role.
Tachykinin-3 Genes and Peptides Characterized in a Basal Teleost, the European Eel: Evolutionary Perspective and Pituitary Role.
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DOI：
10.3389/fendo.2018.00304
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发表时间：
2018
影响因子：
5.2
通讯作者：
Rousseau K
中科院分区：
文献类型：
--
作者：
Campo A;Lafont AG;Lefranc B;Leprince J;Tostivint H;Kamech N;Dufour S;Rousseau K
在哺乳动物中，神经激肽B （NKB）是一种由tac3基因编码的短肽。它主要通过kisspeptin刺激促性腺激素释放激素（GnRH）神经元参与大脑对生殖的控制。我们研究了一种基底硬骨鱼——欧洲鳗的tac3基因和肽，发现它在青春期前的性成熟过程中具有非典型的阻滞作用。在鳗鲡基因组中鉴定出两个tac3亲缘基因（tac3a和tac3b），每个基因编码两个肽（NKBa或b和nkb相关肽NKB-RPa或b）。鳗鱼NKBa的氨基酸序列与人类NKB相同，其余3个是新的肽序列。这四种鳗鱼肽具有特征性的c端速激肽序列，以及相似的α螺旋三维结构。对52种脊椎动物的Tac3基因进行了计算机鉴定，并对预测的Tac3前肽序列进行了系统发育分析。为了进一步了解tac3基因的进化史，我们还对tac3基因进行了同源性分析。硬骨鱼中tac3基因的重复可能是硬骨鱼特异性全基因组复制（3R）的结果。在硬骨鱼中，TAC3b前体序列比TAC3a更具差异性，甚至在某些硬骨鱼谱系中也会出现TAC3b基因的缺失。NKB- rp肽是由tac3基因在放线翼动物和基底肌翼动物中与NKB一起编码的，在祖先羊膜动物中可能已经丢失。通过特异性qpcr分析，鳗鲡tac3a和tac3b mrna的组织分布显示，这两种转录本主要在大脑中表达，尤其是在间脑中。在体外原代培养鳗鱼垂体细胞上对人NKB进行了检测。人NKB剂量依赖性地抑制lhβ的表达，而对其他糖蛋白激素亚基（fshβ、tshβ和gpα）和gh没有影响。人类NKB还能剂量依赖性地抑制GnRH受体（GnRH -r2）的表达。这四种鳗鱼肽已经合成并在体外进行了测试。它们都抑制了lhβ和gnrh-r2的表达。这揭示了由两个tac3基因编码的四种肽在鳗鱼生殖中潜在的双重抑制作用，在垂体水平上对黄体生成素和GnRH受体都有抑制作用。
In mammals, neurokinin B (NKB) is a short peptide encoded by the gene tac3. It is involved in the brain control of reproduction by stimulating gonadotropin-releasing hormone (GnRH) neurons, mainly via kisspeptin. We investigated tac3 genes and peptides in a basal teleost, the European eel, which shows an atypical blockade of the sexual maturation at a prepubertal stage. Two tac3 paralogous genes (tac3a and tac3b) were identified in the eel genome, each encoding two peptides (NKBa or b and NKB-related peptide NKB-RPa or b). Amino acid sequence of eel NKBa is identical to human NKB, and the three others are novel peptide sequences. The four eel peptides present the characteristic C-terminal tachykinin sequence, as well as a similar alpha helix 3D structure. Tac3 genes were identified in silico in 52 species of vertebrates, and a phylogeny analysis was performed on the predicted TAC3 pre-pro-peptide sequences. A synteny analysis was also done to further assess the evolutionary history of tac3 genes. Duplicated tac3 genes in teleosts likely result from the teleost-specific whole genome duplication (3R). Among teleosts, TAC3b precursor sequences are more divergent than TAC3a, and a loss of tac3b gene would have even occurred in some teleost lineages. NKB-RP peptide, encoded beside NKB by tac3 gene in actinopterygians and basal sarcopterygians, would have been lost in ancestral amniotes. Tissue distribution of eel tac3a and tac3b mRNAs showed major expression of both transcripts in the brain especially in the diencephalon, as analyzed by specific qPCRs. Human NKB has been tested in vitro on primary culture of eel pituitary cells. Human NKB dose-dependently inhibited the expression of lhβ, while having no effect on other glycoprotein hormone subunits (fshβ, tshβ, and gpα) nor on gh. Human NKB also dose-dependently inhibited the expression of GnRH receptor (gnrh-r2). The four eel peptides have been synthesized and also tested in vitro. They all inhibited the expression of both lhβ and of gnrh-r2. This reveals a potential dual inhibitory role of the four peptides encoded by the two tac3 genes in eel reproduction, exerted at the pituitary level on both luteinizing hormone and GnRH receptor.
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影响因子：
4.8
作者：
Fraser, Graeme L.;Hoveyda, Hamid R.;Millar, Robert P.
通讯作者：
Millar, Robert P.
DOI：
10.1073/pnas.1119165109
发表时间：
2012-06-26
影响因子：
11.1
作者：
Biran, Jakob;Palevitch, Ori;Levavi-Sivan, Berta
通讯作者：
Levavi-Sivan, Berta
影响因子：
5.8
作者：
Gianetti, Elena;Tusset, Cintia;Seminara, Stephanie B.
通讯作者：
Seminara, Stephanie B.
影响因子：
2.7
作者：
Lecci, Alessandro;Capriati, Angela;Maggi, Carlo Alberto
通讯作者：
Maggi, Carlo Alberto
DOI：
10.3109/10799899509045238
发表时间：
1995-01-01
期刊：
JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH
影响因子：
--
作者：
HENRIKSEN, JS;SAERMARK, T;MAU, SE
通讯作者：
MAU, SE