Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets.
Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets.
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DOI：
10.1038/nnano.2013.194
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发表时间：
2013-10
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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作者：
癌症在全身的扩散是由循环的肿瘤细胞（ctc）驱动的。这些细胞从原发肿瘤中分离出来，从血流中转移到肿瘤生长的新部位。它们还携带有关原发肿瘤的信息，并有可能成为疾病诊断和进展的有价值的生物标志物，以及肿瘤某些生物学特性的分子表征。然而，目前在患者血液样本中测量和研究这些细胞的方法的灵敏度和特异性有限，阻碍了它们充分发挥临床潜力。利用微流控装置分离ctc是一种很有前途的方法；然而，该设备依赖于三维结构，这限制了芯片上细胞的进一步表征和扩展。在这里，我们展示了一种有效的方法，通过在图案金表面上使用功能化氧化石墨烯纳米片，从胰腺癌、乳腺癌和肺癌患者的血液样本中分离ctc。在低浓度靶细胞（3-5个细胞/mL血液）下，ctc捕获灵敏度高（73%±32.4）。
The spread of cancer throughout the body is driven by circulating tumour cells (CTCs). These cells detach from the primary tumour and move from the blood stream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods to measure and study these cells in patient blood samples prevent the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs; however, the devices are reliant on three-dimensional structures, which limit further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolate CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalised graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at low concentration of target cells (73% ± 32.4 at 3–5 cells/mL blood).
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影响因子：
16.6
作者：
Wang, Shutao;Wang, Hao;Jiao, Jing;Chen, Kuan-Ju;Owens, Gwen E.;Kamei, Ken-ichiro;Sun, Jing;Sherman, David J.;Behrenbruch, Christian P.;Wu, Hong;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
9.9
作者：
Sun, Xiaoming;Liu, Zhuang;Dai, Hongjie
通讯作者：
Dai, Hongjie
DOI：
10.1158/1078-0432.ccr-10-1105
发表时间：
2010-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lin HK;Zheng S;Williams AJ;Balic M;Groshen S;Scher HI;Fleisher M;Stadler W;Datar RH;Tai YC;Cote RJ
通讯作者：
Cote RJ
影响因子：
17.1
作者：
Ozkumur E;Shah AM;Ciciliano JC;Emmink BL;Miyamoto DT;Brachtel E;Yu M;Chen PI;Morgan B;Trautwein J;Kimura A;Sengupta S;Stott SL;Karabacak NM;Barber TA;Walsh JR;Smith K;Spuhler PS;Sullivan JP;Lee RJ;Ting DT;Luo X;Shaw AT;Bardia A;Sequist LV;Louis DN;Maheswaran S;Kapur R;Haber DA;Toner M
通讯作者：
Toner M