Characterization of the promoter region of an Arabidopsis gene for 9-cis-epoxycarotenoid dioxygenase involved in dehydration-inducible transcription.
Characterization of the promoter region of an Arabidopsis gene for 9-cis-epoxycarotenoid dioxygenase involved in dehydration-inducible transcription.
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DOI：
10.1093/dnares/dst012
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Shinozaki K
中科院分区：
文献类型：
--
作者：
Behnam B;Iuchi S;Fujita M;Fujita Y;Takasaki H;Osakabe Y;Yamaguchi-Shinozaki K;Kobayashi M;Shinozaki K
植物通过复杂的生理和细胞过程来响应脱水胁迫和耐受缺水状态。许多基因都是由水分亏缺引起的。脱落酸(ABA)通过诱导多种胁迫基因在植物对脱水胁迫的耐受性中发挥重要作用。ABA是在脱水后重新合成的。参与ABA生物合成的基因大部分已被鉴定，并且主要在叶片维管组织中表达。在这类基因的产物中，9-顺式环氧氯丙烷双加氧酶(NCED)是ABA生物合成的关键酶。在拟南芥的5个NCED基因中，AtNCED3基因受到脱水的显著诱导。为了了解脱水应激反应早期的调控机制，分析AtNCED3的转录调控系统是很重要的。在本研究中，我们发现在−转录起始点2248bp处有一个重叠的G盒识别序列(5‘-CACGTG-3’)是诱导脱水反应的一个重要的顺式作用元件。我们讨论了脱水响应AtNCED3表达的可能的转录调控系统，以及在缺水条件下这可能如何控制ABA的水平。
Plants respond to dehydration stress and tolerate water-deficit status through complex physiological and cellular processes. Many genes are induced by water deficit. Abscisic acid (ABA) plays important roles in tolerance to dehydration stress by inducing many stress genes. ABA is synthesized de novo in response to dehydration. Most of the genes involved in ABA biosynthesis have been identified, and they are expressed mainly in leaf vascular tissues. Of the products of such genes, 9-cis-epoxycarotenoid dioxygenase (NCED) is a key enzyme in ABA biosynthesis. One of the five NCED genes in Arabidopsis, AtNCED3, is significantly induced by dehydration. To understand the regulatory mechanism of the early stages of the dehydration stress response, it is important to analyse the transcriptional regulatory systems of AtNCED3. In the present study, we found that an overlapping G-box recognition sequence (5′-CACGTG-3′) at −2248 bp from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response. We discuss the possible transcriptional regulatory system of dehydration-responsive AtNCED3 expression, and how this may control the level of ABA under water-deficit conditions.
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影响因子：
7.2
作者：
Umezawa, T;Okamoto, M;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
7.3
作者：
Thompson, Andrew J.;Mulholland, Barry J.;Taylor, Ian B.
通讯作者：
Taylor, Ian B.
DOI：
10.1016/j.bbrc.2003.11.119
发表时间：
2004-01-09
影响因子：
3.1
作者：
Urano, K;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
3.5
作者：
Urano, K;Hobo, T;Shinozaki, K
通讯作者：
Shinozaki, K
DOI：
10.1073/pnas.96.26.15354
发表时间：
1999-12-21
影响因子：
11.1
作者：
Qin, XQ;Zeevaart, JAD
通讯作者：
Zeevaart, JAD