A genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS).
A genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS).
复制标题
使用限制性标志基因组扫描 (RLGS) 绘制的小鼠遗传连锁图。
DOI：
10.1093/genetics/138.4.1207
复制
发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Shiroishi,T
中科院分区：
文献类型：
--
作者：
Hayashizaki,Y;Hirotsune,S;Okazaki,Y;Shibata,H;Akasako,A;Muramatsu,M;Kawai,J;Hirasawa,T;Watanabe,S;Shiroishi,T
我们开发了一种多重基因组分析方法，限制性地标基因组扫描(RLGS)，已用于构建小鼠的遗传图谱。限制标志是基因组DNA的末端标记限制片段，通过使用高分辨率的双向凝胶电泳法分离，在单个凝胶中识别多达2000个标志基因。已经在实验室菌株之间以及这些菌株和小家鼠之间鉴定了数百个这些基因座的变异。对1100多个RLGS基因座在重组自交系和两个不同种间遗传杂交中的分离进行了分析。已经得到了将1045个RLGS基因座与小鼠基因组的所有常染色体和X染色体上的参考基因座相关联的遗传图谱。RLGS方法可以应用于许多不同生物的基因组分析，以确定基因组位置，因为它使用限制性标志物的末端标记，而不是探针杂交。不同的限制性内切酶组合产生了不同的RLGS基因座，为遗传图谱提供了更大的力量。
We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled restriction fragments of genomic DNA that are separated by using high resolution, two-dimensional gel electrophoresis identifying as many as two thousand landmark loci in a single gel. Variation for several hundred of these loci has been identified between laboratory strains and between these strains and Mus spretus. The segregation of more than 1100 RLGS loci has been analyzed in recombinant inbred (RI) strains and in two separate interspecific genetic crosses. Genetic maps have been derived that link 1045 RLGS loci to reference loci on all of the autosomes and the X chromosome of the mouse genome. The RLGS method can be applied to genome analysis in many different organisms to identify genomic loci because it uses end-labeling of restriction landmarks rather than probe hybridization. Different combinations of restriction enzymes yield different sets of RLGS loci providing expanded power for genetic mapping.
影响因子：
56.9
作者：
COPELAND, NG;JENKINS, NA;LANDER, ES
通讯作者：
LANDER, ES