Development of choroidal neovascularization in rats with advanced intense cyclic light-induced retinal degeneration.
Development of choroidal neovascularization in rats with advanced intense cyclic light-induced retinal degeneration.
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DOI：
10.1001/archophthalmol.2009.395
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发表时间：
2010-02
影响因子：
--
通讯作者：
Sheibani, Nader
中科院分区：
文献类型：
--
作者：
Albert, Daniel M.;Neekhra, Aneesh;Wang, Shoujian;Darjatmoko, Soesiawati R.;Sorenson, Christine M.;Dubielzig, Richard R.;Sheibani, Nader
目的：研究强周期性光诱导的视网膜变性的进行性变化，并确定其是否导致脉络膜新生血管(CNV)。白化大鼠暴露于3000Lux周期光12h，时间分别为1、3、6个月。安乐死前进行眼底检查、眼底照片、荧光素和吲哚青绿血管造影以及光学相干断层扫描(OCT)评估。光照后1、3或6个月处死动物，进行组织病理学评价。免疫荧光染色检测视网膜4-羟基-2-壬烯醛(HNE)和硝基酪氨酸修饰蛋白的存在。1个月后，慢性强光暴露导致视网膜变性，光感受器外节和约三分之二的外核层(ONL)丢失，视网膜下色素上皮(RPE)新生血管形成。3个月后，几乎整个ONL消失，CNV穿透Bruch膜并延伸到视网膜外。6个月后观察到无ONL、CNV多灶性、RPE纤维化生和含有血管伸入视网膜的结缔组织带。所有强光暴露的动物均表现出HNE和硝基酪氨酸染色的增加。OCT和血管造影检查证实新形成的血管视网膜变薄和渗漏。我们的结果提示，白化大鼠在慢性强周期光暴露后会出现视网膜退行性变和CNV的进行性阶段，这为详细研究老年性黄斑变性的发病机制和治疗提供了可能。
To study the progressive changes of intense cyclic light-induced retinal degeneration and determine whether it results in choroidal neovascularization (CNV). Albino rats were exposed to 12 h of 3000 lux cyclic light for 1, 3, or 6 months. Prior to euthanization, fundus examination, fundus photographs, fluorescein and indocyanine green angiography, and Optical Coherence Tomography (OCT) evaluations were performed. Light exposed animals were euthanized after 1, 3, or 6 months for histopathological evaluation. Retinas were examined for the presence of 4-hydroxy-2-nonenal (HNE) and nitrotyrosine modified proteins by immunofluorescence staining. Chronic intense cyclic light exposure resulted in retinal degeneration with loss of the outer segments of photoreceptors and approximately two-thirds of the outer nuclear layer (ONL) and development of sub-retinal pigment epithelium (RPE) neovascularization after 1 month. Almost the entire ONL was absent with the presence of CNV, which penetrated Bruch’s membrane and extended into the outer retina after 3 months. Absence of the ONL, multiple foci of CNV, RPE fibrous metaplasia, and connective tissue bands containing blood vessels extending into the retina were observed after 6 months. All intense light exposed animals showed an increased presence of HNE and nitrotyrosine staining. OCT and angiographic studies confirmed retinal thinning and leakiness of the newly fromed blood vessels. Our results suggest albino rats develop progressive stages of retinal degeneration and CNV after chronic intense cyclic light exposure allowing the detailed study of the pathogenesis and treatment of age-related macular degeneration.
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影响因子：
4.1
作者：
Evans, J;Wormald, R
通讯作者：
Wormald, R
影响因子：
158.5
作者：
Eremina, Vera;Jefferson, J. Ashley;Quaggin, Susan E.
通讯作者：
Quaggin, Susan E.
影响因子：
--
作者：
CRUICKSHANKS, KJ;KLEIN, R;KLEIN, BEK
通讯作者：
KLEIN, BEK
影响因子：
5.6
作者：
Dong, Aling;Xie, Bing;Shen, Jikui;Yoshida, Tsunehiko;Yokoi, Katsutoshi;Hackett, Sean F.;Campochiaro, Peter A.
通讯作者：
Campochiaro, Peter A.
影响因子：
4.8
作者：
Donovan, M;Carmody, RJ;Cotter, TG
通讯作者：
Cotter, TG