Contralateral paw sensitization following injection of endothelin-1: effects of local anesthetics differentiate peripheral and central processes.
Contralateral paw sensitization following injection of endothelin-1: effects of local anesthetics differentiate peripheral and central processes.
复制标题
DOI：
10.1016/j.neuroscience.2009.10.049
复制
发表时间：
2010-01-20
期刊：
影响因子：
3.3
通讯作者：
Strichartz, G. R.
中科院分区：
文献类型：
--
作者：
Khodorova, A.;Strichartz, G. R.
已知将肽内皮素-1（ET-1）皮下注射到大鼠的足垫中会引起快速、短暂的同侧机械和热致敏以及伤害性后爪退缩。在这里，我们报告说，局部注射ET-1（2纳摩尔）到一个后爪缓慢敏感的对侧爪化学和机械刺激。与第一次注射相比，第二次注射相同剂量的ET-1 24小时后递送到对侧爪中，后爪退缩反应增加1.5-2倍。ET-1后24小时，对侧爪也出现了对福尔马林反应第二阶段期间退缩次数的类似增加。在同侧ET-1注射后24 h，对侧对von Frey毛的缩爪阈值降低约55%。ET-1皮下注射在一个节段无关的位置，颈部中线，没有引起爪致敏，避免了全身性的作用途径。局部麻醉阻滞同侧坐骨神经在同侧ET-1的初始反应期间防止对侧敏化，表明局部传入传递的重要性，虽然同侧脱敏没有改变。这些研究结果表明，外周ET-1的行动导致中央敏化，改变了选定的刺激反应。
Subcutaneous injection of the peptide endothelin-1 (ET-1) into the rat’s footpad is known to cause rapid, transient ipsilateral mechanical and thermal sensitization and nocifensive hind paw flinching. Here we report that local injection of ET-1 (2 nmoles) into one hind paw slowly sensitizes the contralateral paw to chemical and mechanical stimulation. There was a 1.5–2-fold increase in the hind paw flinching response, over that from the first injection, to a second injection of the same dose of ET-1 delivered 24 h later into the contralateral paw. A similar increase in the number of flinches during the second phase of the response to formalin also occurred in the contralateral paw 24 h after ET-1. The contralateral paw withdrawal threshold to von Frey hairs was lowered by ~ 55% at 24 h after ipsilateral ET-1 injection. ET-1 injected subcutaneously at a segmentally unrelated location, the nuchal midline, caused no sensitization of the paws, obviating a systemic route of action. Local anesthetic block of the ipsilateral sciatic nerve during the period of initial response to ipsilateral ET-1 prevented contralateral sensitization, indicating the importance of local afferent transmission, although ipsilateral desensitization was not changed. These findings suggest that peripheral ET-1 actions lead to central sensitization that alters responses to selected stimuli.
登录
查看更多内容
影响因子：
4.8
作者：
Bremnes, T;Paasche, JD;Attramadal, H
通讯作者：
Attramadal, H
影响因子：
3.3
作者：
LESLIE, TA;EMSON, PC;WOOLF, CJ
通讯作者：
WOOLF, CJ
影响因子：
8.8
作者：
Gokin, AP;Philip, B;Strichartz, GR
通讯作者：
Strichartz, GR
影响因子：
8.8
作者：
LEE, KC;WILDER, RT;BERDE, CB
通讯作者：
BERDE, CB
影响因子：
3
作者：
CYR, C;KRIS, RM
通讯作者：
KRIS, RM