Staphylococcus aureus Exploits Epidermal Barrier Defects in Atopic Dermatitis to Trigger Cytokine Expression.
Staphylococcus aureus Exploits Epidermal Barrier Defects in Atopic Dermatitis to Trigger Cytokine Expression.
复制标题
DOI：
10.1016/j.jid.2016.05.127
复制
发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Gallo RL
中科院分区：
文献类型：
--
作者：
Nakatsuji T;Chen TH;Two AM;Chun KA;Narala S;Geha RS;Hata TR;Gallo RL
特应性皮炎（AD）患者的皮肤屏障功能异常，并经常被沙门氏菌定植。金黄色。在这项研究中，我们调查了S.金黄色葡萄球菌穿透AD受试者的表皮屏障，并试图了解这种进入的机制和功能意义。S.金黄色葡萄球菌在AD患者皮损皮肤的真皮中更丰富。在培养的人类皮肤等同物和小鼠中观察到细菌通过表皮进入，但发现在凯萨林菌素敲除（Camp-/-）和卵清蛋白致敏的聚丝蛋白突变体（FLGft/ft）小鼠的皮肤中增加。S.金黄色葡萄球菌穿透表皮依赖于细菌活力和蛋白酶活性，如杀死的细菌或S.金黄色葡萄球菌无法穿透。S.金黄色葡萄球菌的表达与IL 4、IL 13、IL 22、TSLP和其他与AD相关的细胞因子的表达增加以及cathelicidin的表达减少直接相关。这些数据说明了AD中表皮屏障的异常如何改变S。金黄色葡萄球菌进入真皮，并提供了这样的皮肤生态失调如何导致增加的炎性细胞因子和疾病恶化的解释。
Patients with atopic dermatitis (AD) have an abnormal skin barrier and are frequently colonized by S. aureus. In this study we investigated if S. aureus penetrates the epidermal barrier of subjects with AD and sought to understand the mechanism and functional significance of this entry. S. aureus was observed to be more abundant in the dermis of lesional skin from AD patients. Bacterial entry past the epidermis was observed in cultured human skin equivalents and in mice, but found to be increased in the skin of cathelicidin knockout (Camp−/−) and ovalbumin-sensitized filaggrin mutant (FLGft/ft) mice. S. aureus penetration through the epidermis was dependent on bacterial viability and protease activity as killed bacteria or a protease-null mutant strain of S. aureus was unable to penetrate. Entry of S. aureus directly correlated with increased expression of IL4, IL13, IL22, TSLP and other cytokines associated with AD, and with decreased expression of cathelicidin. These data illustrate how abnormalities of the epidermal barrier in AD can alter the balance of S. aureus entry into the dermis and provides an explanation for how such dermal dysbiosis results in increased inflammatory cytokines and exacerbation of disease.
登录
查看更多内容
影响因子：
14.2
作者：
De Benedetto, Anna;Rafaels, Nicholas M;McGirt, Laura Y;Ivanov, Andrei I;Georas, Steve N;Cheadle, Chris;Berger, Alan E;Zhang, Kunzhong;Vidyasagar, Sadasivan;Yoshida, Takeshi;Boguniewicz, Mark;Hata, Tissa;Schneider, Lynda C;Hanifin, Jon M;Gallo, Richard L;Novak, Natalija;Weidinger, Stephan;Beaty, Terri H;Leung, Donald Y M;Barnes, Kathleen C;Beck, Lisa A
通讯作者：
Beck, Lisa A
影响因子：
4.4
作者：
Bekeredjian-Ding, Isabelle;Inamura, Seiichi;Hartmann, Gunther
通讯作者：
Hartmann, Gunther
DOI：
10.1073/pnas.91.23.11035
发表时间：
1994-11-08
影响因子：
11.1
作者：
GALLO, RL;ONO, M;BERNFIELD, M
通讯作者：
BERNFIELD, M
影响因子：
56.9
作者：
Liu, PT;Stenger, S;Modlin, RL
通讯作者：
Modlin, RL
皮肤共生体中的一种新型脂肽可激活 TLR2/CD36-p38 MAPK 信号传导以增强针对细菌感染的抗菌防御
影响因子：
3.7
作者：
Li D;Lei H;Li Z;Li H;Wang Y;Lai Y
通讯作者：
Lai Y