Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.
Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.
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DOI：
10.1038/sj.bjc.6600307
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发表时间：
2002-06-05
影响因子：
8.8
通讯作者：
Maehara, T
中科院分区：
文献类型：
--
作者：
Ono, K;Ishihara, M;Ishikawa, K;Ozeki, Y;Deguchi, H;Sato, M;Hashimoto, H;Saito, Y;Yura, H;Kurita, A;Maehara, T
关键词：
高碘酸盐处理的非抗凝肝素载聚苯乙烯由约10个高碘酸盐氧化、碱降解的低分子量肝素链连接到聚苯乙烯核组成，其抗凝活性明显低于肝素。在这项研究中，我们评估了非抗凝肝素载聚苯乙烯对肿瘤生长和转移的影响。非抗凝肝素载聚苯乙烯对血管内皮生长因子-165-、成纤维细胞生长因子-2-或肝细胞生长因子诱导的人微血管内皮细胞生长的抑制活性高于肝素、10-高碘酸氧化-肝素和10-高碘酸氧化-低分子量-肝素，这可能是由于非抗凝肝素载聚苯乙烯的肝素聚集效应。非抗凝肝素载聚苯乙烯抑制人微血管内皮细胞，B16黑色素瘤和刘易斯肺癌细胞粘附到Matrigel包被的板。非抗凝肝素载聚苯乙烯在基质胶包被小室试验中对基质胶和B16-黑色素瘤的内皮细胞小管形成和刘易斯肺癌细胞侵袭也显示出强抑制活性。体内研究表明，非抗凝肝素载聚苯乙烯比10个高碘酸氧化肝素和10个高碘酸氧化低分子量肝素更有效地抑制皮下诱导的肿瘤生长和B16-黑色素瘤和刘易斯肺癌细胞的肺转移。此外，非抗凝肝素载聚苯乙烯显着减少了皮下刘易斯肺癌肿瘤中的CD 34阳性血管的数量，表明对血管生成的强烈抑制。这些结果表明，非抗凝肝素载聚苯乙烯具有抑制血管生成和肿瘤侵袭的活性，可能是非常有用的癌症治疗。英国癌症杂志（2002）86，1803-1812。www.bjcancer.com © 2002英国癌症研究中心
Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin. In this study, we evaluated the effect of non-anticoagulant heparin-carrying polystyrene on tumour growth and metastasis. Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene. Non-anticoagulant heparin-carrying polystyrene inhibited human microvascular endothelial cell, B16 melanoma and Lewis lung cancer cell adhesion to Matrigel-coated plates. Non-anticoagulant heparin-carrying polystyrene also showed strong inhibitory activities in the tubular formation of endothelial cells on Matrigel and B16-melanoma and Lewis lung cancer cell invasion in a Matrigel-coated chamber assay. In vivo studies showed that growth of subcutaneous induced tumours and lung metastasis of B16-melanoma and Lewis lung cancer cells were more effectively inhibited by non-anticoagulant heparin-carrying polystyrene than ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin. Furthermore, non-anticoagulant heparin-carrying polystyrene markedly reduced the number of CD34-positive vessels in subcutaneous Lewis lung cancer tumours, indicating a strong inhibition of angiogenesis. These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy. British Journal of Cancer (2002) 86, 1803–1812. doi:10.1038/sj.bjc.6600307 www.bjcancer.com © 2002 Cancer Research UK
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影响因子：
7.5
作者：
Basbaum, CB;Werb, Z
通讯作者：
Werb, Z
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Ishihara, M;Ono, K;Kurita, A
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