Campylobacter jejuni uses energy taxis and a dehydrogenase enzyme for l-fucose chemotaxis.
Campylobacter jejuni uses energy taxis and a dehydrogenase enzyme for l-fucose chemotaxis.
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空肠弯曲杆菌是全球细菌性腹泻的主要原因，在低收入和中等收入国家，婴儿感染与生长发育迟缓有关。大约一半的空肠梭菌分离株具有解糖功能，而其余的则编码能够进行单点分解代谢的酶。我们之前的研究表明，来自撒哈拉以南非洲和南亚的母乳喂养婴儿与解糖菌株相比，l-焦代谢空肠梭菌的定植频率较低。我们还表明，FucX是一种l-聚焦脱氢酶，即使在没有l-聚焦的情况下，它也能与NADP+辅助因子结合，并且当将该酶引入缺乏代谢途径的菌株时，该酶足以实现l-聚焦趋化。该研究表明，FucX可能通过增加细胞NADPH/NADP+比率来影响l-聚焦趋化性，从而影响能量趋化成分CetABC，该成分响应电子受体和供体梯度的移动。此外，空肠C. CetAB和CetAC对大肠杆菌的致氧性有补充作用。我们的数据支持一个模型，即NADP+水平的变化受FucX脱氢酶的影响，影响l-聚焦反应的趋化性。这种游动行为可以用多佛氏伯克氏菌同源的l-病灶脱氢酶进行表型复制。综上所述，我们的工作提供了一种可能的解释，为什么l- focal代谢空肠梭菌分离物从肠上皮细胞游向管腔内的自由病灶，随后被母乳喂养的婴儿清除。在这项研究中，我们确定了空肠弯曲杆菌l-灶脱氢酶在l-灶趋化性中的单独作用，并证明这种机制不仅限于空肠弯曲杆菌，而且存在于多佛伯克霍尔德氏菌中。我们现在假设，l-聚焦能量趋向性可能有助于减少母乳喂养婴儿胃肠道中l-聚焦代谢的空肠梭菌菌株，选择具有增加定植潜力的分离株。
Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and infection in infants is associated with growth stunting in low- and middle-income countries. Approximately half of all C. jejuni isolates are asaccharolytic, while the remainder encode enzymes capable of l-fucose catabolism. Our previous study suggested that breastfed infants originating from sub-Saharan Africa and South Asia were colonized less frequently with l-fucose-metabolizing C. jejuni isolates compared to asaccharolytic strains. We also showed that FucX is an l-fucose dehydrogenase that binds to its NADP+ cofactor even in the absence of l-fucose and that this enzyme is sufficient for l-fucose chemotaxis when introduced into strains lacking the metabolic pathway. This study indicates that FucX may influence l-fucose chemotaxis by increasing cellular NADPH/NADP+ ratios, which subsequently affect the energy taxis components, CetABC that respond to shifting gradients of electron acceptors and donors. Furthermore, C. jejuni CetAB and CetAC can complement Escherichia coli aerotaxis. Our data support a model in which the shift in NADP+ levels, impacted by the FucX dehydrogenase, affects chemotaxis in response to l-fucose. This swimming behavior can be phenocopied with a homologous l-fucose dehydrogenase from Burkholderia multivorans. Taken together, our work provides a possible explanation for why l-fucose-metabolizing C. jejuni isolates swim away from intestinal epithelial cells toward free fucose in the lumen where they are subsequently cleared by breastfed infants. In this study, we identify a separate role for the Campylobacter jejuni l-fucose dehydrogenase in l-fucose chemotaxis and demonstrate that this mechanism is not only limited to C. jejuni but is also present in Burkholderia multivorans. We now hypothesize that l-fucose energy taxis may contribute to the reduction of l-fucose-metabolizing strains of C. jejuni from the gastrointestinal tract of breastfed infants, selecting for isolates with increased colonization potential.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
埃塞俄比亚农村幼儿的弯曲杆菌定植，环境肠功能障碍，发育迟缓和相关危险因素：弯曲杆菌基因组学和环境肠功能障碍（CAIGED）项目的横断面研究。
影响因子：
5.2
作者：
Chen D;McKune SL;Singh N;Yousuf Hassen J;Gebreyes W;Manary MJ;Bardosh K;Yang Y;Diaz N;Mohammed A;Terefe Y;Roba KT;Ketema M;Ameha N;Assefa N;Rajashekara G;Deblais L;Ghanem M;Yimer G;Havelaar AH
通讯作者：
Havelaar AH
影响因子：
3.2
作者：
Nasher F;Taylor AJ;Elmi A;Lehri B;Ijaz UZ;Baker D;Goram R;Lynham S;Singh D;Stabler R;Kelly DJ;Gundogdu O;Wren BW
通讯作者：
Wren BW
影响因子：
168.9
作者：
Lozano, Rafael;Naghavi, Mohsen;Murray, Christopher J. L.
通讯作者：
Murray, Christopher J. L.
CetA 和 CetB（空肠弯曲杆菌中的双向能量趋向系统）的表征。
影响因子：
3.6
作者：
Elliott KT;Dirita VJ
通讯作者：
Dirita VJ