Do heat shock proteins provide protection against freezing?
Do heat shock proteins provide protection against freezing?
复制标题
热休克蛋白能提供防冻保护吗？
作者：
Yasahiko Komatsu;Sunil C. Kaul;Hitoshi Iwahashi;K. Obuchi
酵母细胞在43℃暴露后直接投入液氮(LN2)进行冷冻，未经热休克冷冻的细胞和经放线菌亚胺处理的细胞在冷冻和解冻过程中几乎100%丧失活力。冷冻和解冻前的热暴露显著提高了细胞的活力。这种细胞活力的增加与热休克蛋白合成的诱导有关，凝胶电泳法检测到这一点。这种蛋白质可能通过稳定大分子和增加疏水相互作用来发挥作用。
Yeast cells were frozen by plunging directly into liquid nitrogen (LN2) after exposure at 43 degrees C. Both the cells frozen without prior exposure to heat shock and those treated with cycloheximide showed almost 100% loss of viability during freezing and thawing. Heat exposure prior to freezing and thawing significantly increased the cell viability. This increase in cell viability was associated with the induction of heat shock protein synthesis, which was detected by gel electrophoresis. This protein may act by stabilizing the macromolecules and by increasing the hydrophobic interactions.
一元醇和多羟基醇与 CHO 细胞中高温的相互作用。
影响因子：
3.4
作者：
Henle,KJ
通讯作者：
Henle,KJ
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z
在体外防止多元醇热诱导的细胞杀伤。
影响因子：
11.2
作者：
Henle,KJ;Peck,JW;Higashikubo,R
通讯作者：
Higashikubo,R