How Bacteria Adhere to Brushy PEG Surfaces: Clinging to Flaws and Compressing the Brush.
How Bacteria Adhere to Brushy PEG Surfaces: Clinging to Flaws and Compressing the Brush.
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DOI：
10.1021/ma300981r
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发表时间：
2012-10-23
期刊：
影响因子：
5.5
通讯作者：
Santore MM
中科院分区：
文献类型：
--
作者：
Gon S;Kumar KN;Nüsslein K;Santore MM
这项研究考察了在金黄色葡萄球菌(S.aureus)从缓流中粘连的初始阶段，生物工程表面上的溶剂化聚合物刷子的压缩情况。一系列高度为7~17 nm、对蛋白质和细菌完全无粘附性的聚乙二醇型聚乙二醇刷，在其底部加入了稀疏的、孤立的~10 nm阳离子聚合物“贴片”，对其进行了修饰。这些缺乏聚乙二醇链的纳米区域对负性细菌或蛋白质具有静电吸引力。由多个粘合剂贴片绘制到界面上的金黄色葡萄球菌将聚乙二醇刷压缩在剩余接触区域。观察到的细菌或纤维蛋白原捕获随着斑块含量增加而开始的情况与计算结果进行了比较。平衡吸引能量(与捕获过程中与细菌接触的贴片数量成正比)与空间作用力(使用Alexander-DeGennes处理计算)提供了关于刷子压缩的视角。在这些研究中，结果与德拜长度数量级的细菌-表面间隙一致。在这种强大的刷子压缩极限下，刷子的结构特征(高度、持续长度)并不重要，因此渗透压主导着空间斥力。因此，细菌排斥的主要因素是刷子中聚乙二醇的质量。这一结果解释了文献中的经验报告，这些报告将刷子的总PEG含量确定为防止生物粘连的标准，与系绳长度和间距无关，在这些参数的合理范围内。细菌捕获法与蛋白质捕获法也进行了比较。令人惊讶的是，这种斑状刷子更能抵抗蛋白质，而不是细菌。金黄色葡萄球菌的粘附性是由耐蛋白质的聚乙二醇酯刷子内的斑块驱动的，这可以解释为细菌更倾向于压缩大片的刷子与许多斑块相互作用。相比之下，蛋白质被认为穿透了几个不含PEO的斑块的刷子。这一发现为文献报道提供了一个机制，即体外蛋白质抵抗不能很好地预测与细胞-表面黏附相关的体外植入失败。
This study examined the compression of solvated polymer brushes on bioengineered surfaces during the initial stages of Staphylococcus Aureus (S. aureus) adhesion from gentle flow. A series of PEG [poly(ethylene glycol)] brushes, 7 to 17 nm in height and completely non-adhesive to proteins and bacteria, were modified by the incorporation of sparse isolated ~10 nm cationic polymer “patches” at their bases. These nanoscale regions, which lacked PEG tethers, were electrostatically attractive towards negative bacteria or proteins. S. aureus drawn to the interface by multiple adhesive patches compressed the PEG brush in the remaining contact region. The observed onset of bacterial or fibrinogen capture with increases in patch content was compared with calculations. Balancing the attraction energy (proportional to the number of patches engaging a bacterium during capture) against steric forces (calculated using the Alexander-DeGennes treatment) provided perspective on the brush compression. The results were consistent with a bacteria-surface gap on the order of the Debye length in these studies. In this limit of strong brush compression, structural features (height, persistence length) of the brush were unimportant so that osmotic pressure dominated the steric repulsion. Thus, the dominant factor for bacterial repulsion was the mass of PEG in the brush. This result explains empirical reports in the literature that identify the total PEG content of a brush as a criteria for prevention of bioadhesion, independent of tether length and spacing, within a reasonable range for those parameters. Bacterial capture was also compared to that of protein capture. It was found, surprisingly, that the patchy brushes were more protein-than bacteria-resistant. S. aureus adhesion driven by patches within otherwise protein-resistant PEG brushes was explained by the bacteria’s greater tendency to compress large areas of brush to interact with many patches. By contrast, proteins are thought to penetrate the brush at a few sites of PEO-free patches. The finding provides a mechanism for the literature reports that in-vitro protein resistance is a poor predictor of in-vitro implant failure related to cell-surface adhesion.
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影响因子：
3.9
作者：
Halperin, A
通讯作者：
Halperin, A
影响因子：
3.4
作者：
Heuberger, M;Drobek, T;Spencer, ND
通讯作者：
Spencer, ND
影响因子：
9.9
作者：
Duffadar, Ranojoy;Kalasin, Surachate;Santore, Maria M.
通讯作者：
Santore, Maria M.
DOI：
10.1016/s0927-7757(97)00218-5
发表时间：
1998-04-15
影响因子：
5.2
作者：
Fu, ZG;Santore, MM
通讯作者：
Santore, MM
影响因子：
3.9
作者：
Gon, Saugata;Santore, Maria M.
通讯作者：
Santore, Maria M.