DNA-launched live-attenuated vaccines for biodefense applications.
DNA-launched live-attenuated vaccines for biodefense applications.
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DOI：
10.1080/14760584.2016.1175943
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发表时间：
2016-09
影响因子：
6.2
通讯作者：
Tretyakova I
中科院分区：
文献类型：
--
作者：
Pushko P;Lukashevich IS;Weaver SC;Tretyakova I
一种新型疫苗平台利用DNA免疫在体内推出减毒活疫苗。该技术已应用于针对具有全球公共卫生影响的正链RNA病毒（包括甲病毒和黄病毒）的疫苗开发。dna启动疫苗代表重组质粒，编码真核生物启动子下游减毒活病毒的全长基因组RNA。当在体内给药时，减毒活病毒的基因组RNA被转录。RNA在疫苗接受者的组织中启动基因定义的减毒活疫苗病毒的有限复制，从而诱导保护性免疫反应。该平台结合了反向遗传学、DNA免疫和减毒活疫苗的优势，从而减少了基因逆转的机会，提高了安全性，并改善了免疫。有了这种疫苗技术，DNA疫苗的领域从那些表达亚基抗原的疫苗扩展到包括一种新型的DNA疫苗，可以发射减毒活病毒。
A novel vaccine platform uses DNA immunization to launch live-attenuated virus vaccines in vivo. This technology has been applied for vaccine development against positive-strand RNA viruses with global public health impact including alphaviruses and flaviviruses. The DNA-launched vaccine represents the recombinant plasmid that encodes the full-length genomic RNA of live-attenuated virus downstream from a eukaryotic promoter. When administered in vivo, the genomic RNA of live-attenuated virus is transcribed. The RNA initiates limited replication of a genetically defined, live-attenuated vaccine virus in the tissues of the vaccine recipient, thereby inducing a protective immune response. This platform combines the strengths of reverse genetics, DNA immunization and the advantages of live-attenuated vaccines, resulting in a reduced chance of genetic reversions, increased safety, and improved immunization. With this vaccine technology, the field of DNA vaccines is expanded from those that express subunit antigens to include a novel type of DNA vaccines that launch live-attenuated viruses.
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影响因子：
5.4
作者：
Bhat N;Fitzgerald KA
通讯作者：
Fitzgerald KA
DOI：
10.1073/pnas.1111650108
发表时间：
2011-09-20
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
5.4
作者：
Gorchakov, Rodion;Wang, Eryu;Weaver, Scott C.
通讯作者：
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影响因子：
5.5
作者：
Haber, Penina;Moro, Pedro L.;Shimabukuro, Tom T.
通讯作者：
Shimabukuro, Tom T.
影响因子：
5.4
作者：
Cheng, Benson Yee Hin;Ortiz-Riano, Emilio;Martinez-Sobrido, Luis
通讯作者：
Martinez-Sobrido, Luis