Differential effect of weight loss on adipocyte size subfractions in patients with type 2 diabetes.
Differential effect of weight loss on adipocyte size subfractions in patients with type 2 diabetes.
复制标题
体重减轻对2型糖尿病患者脂肪细胞大小亚分离的差异影响。
DOI：
10.1038/oby.2009.219
复制
发表时间：
2009-10
期刊：
影响因子：
6.9
通讯作者：
Ravussin, Eric
中科院分区：
文献类型：
--
作者：
Pasarica, Magdalena;Tchoukalova, Yourka D.;Heilbronn, Leonie K.;Fang, Xiaobing;Albu, Jeanine B.;Kelley, David E.;Smith, Smith R.;Ravussin, Eric
脂肪细胞的大小影响它们的功能，这表明对干预的反应不同。我们假设，2型糖尿病(T2 DM)患者的体重减轻主要是减少了大的和非常大的脂肪细胞亚组分的大小，同时伴随着血清脂肪因子的有益变化和胰岛素敏感性的改善。糖尿病患者的Look Action for Health试验共有44名志愿者，他们在经过一年的高强度生活方式干预后体重减轻。测量胰岛素敏感性(高胰岛素-正常血糖钳夹)、腹部皮下脂肪细胞大小(Os固定)和选定的血清脂肪因子。13%的体重减轻伴随着46%的胰岛素敏感性改善(葡萄糖处理率从5.9±2.2 mg/min/kg脱脂质量增加到8.6±2.7 mg/min/kg脱脂质量，P<；0.0 5)，同时血浆脂联素浓度增加36%(6.1±3.1至8.3±3.9µg/ml，P<；0.0 5)，但促炎细胞因子IL-6和肿瘤坏死因子-α没有变化。脂联素的变化与葡萄糖处理率的变化相关(r=0.34，P<；0.05)。平均脂肪细胞大小减少(0.84±0.25至0.64±0.23μL，P<；0.05)，主要是由于大小脂肪细胞亚组分的变化(大小为0.75-0.44微米L，相对数量为19-26%；P<；0.05)。我们的数据表明，大脂肪细胞亚组分的改变可能通过增加血清脂联素来改善胰岛素敏感性。这种关系并不意味着因果关系，不能仅通过测量脂肪细胞的平均大小来获得。这些数据为未来研究中脂肪细胞大小分布与体外脂肪因子分泌和脂解作用相一致的测量提供了支持。
The size of adipocytes influences their function suggesting a differential responsiveness to intervention. We hypothesized that weight loss in patients with type 2 diabetes mellitus (T2DM) predominantly decreases the size of large and very-large adipocyte subfractions in parallel with beneficial changes in serum adipokines and improved insulin sensitivity. A total of 44 volunteers from the Look Action for Health in Diabetes trial, who lost weight after 1-year of intense lifestyle intervention, were included. Insulin sensitivity (hyperinsulinemic–euglycemic clamp), size of subcutaneous abdominal adipocytes (osmium fixation), and selected serum adipokines were measured. A 13% weight loss was accompanied by 46% improvement in insulin sensitivity (increased glucose disposal rate from 5.9 ± 2.2 to 8.6 ± 2.7 mg/min/kg fat-free mass, P < 0.05) in parallel with a 36% increase in plasma adiponectin concentration (6.1 ± 3.1 to 8.3 ± 3.9 µg/ml, P < 0.05], but no changes in the proinflammatory cytokines interleukin-6 and tumor necrosis factor-α. Change in adiponectin correlated with changes in glucose disposal rate (r = 0.34, P < 0.05). Mean adipocyte size decreased (0.84 ± 0.25 to 0.64 ± 0.23 μl, P < 0.05), mainly due to changes in the large adipocyte subfraction (size 0.75–0.44 µl, relative number 19–26%; P < 0.05). Our data suggest that change in the large adipocyte subfraction may contribute to the improvement in insulin sensitivity via an increase in serum adiponectin. Such a relationship, which does not imply cause and effect, could not be obtained by measuring only mean adipocyte size. These data provide support for the measures of adipocyte size distribution in concert with in vitro adipokine secretion and lipolysis in future studies.
登录
查看更多内容
影响因子：
16.2
作者：
Shadid, S;Jensen, MD
通讯作者：
Jensen, MD
影响因子：
4.8
作者：
Blüher, M;Wilson-Fritch, L;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
5.4
作者：
Bahceci, M.;Gokalp, D.;Arikan, S.
通讯作者：
Arikan, S.
影响因子：
7.7
作者：
Hotta, K;Funahashi, T;Matsuzawa, Y
通讯作者：
Matsuzawa, Y
影响因子：
3.9
作者：
de Cabo, R;Fürer-Galbán, S;Lane, MA
通讯作者：
Lane, MA