Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment.
Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment.
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利用水芹绿色合成金纳米颗粒及其生化表征和评估
DOI：
10.3390/nano12010028
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发表时间：
2021-12-23
期刊：
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Hutchinson N;Wu Y;Wang Y;Kanungo M;DeBruine A;Kroll E;Gilmore D;Eckrose Z;Gaston S;Matel P;Kaltchev M;Nickel AM;Kumpaty S;Hua X;Zhang W
本研究的重点是植物介导的绿色合成过程，利用旱地芥（barbararea verna）生产金纳米粒子（Au NPs），因为旱地芥中的各种生物分子既可以作为还原剂，也可以作为capping剂。利用紫外可见光谱、表面电荷（zeta电位）分析、扫描电子显微镜-能量色散x射线能谱（SEM-EDX）、原子力显微镜（AFM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和x射线衍射（XRD）对合成的金纳米粒子进行了全面表征。结果表明，合成的金纳米粒子呈球形，分散良好，平均直径约11 nm，特征吸光度峰约529 nm。EDX结果显示，金含量为11.13%。胶体Au NP稳定性得到证实，ζ电位（ζ）值为−36.8 mV。x射线衍射分析证实了面心立方金结晶的产生。此外，用革兰氏阴性大肠杆菌和革兰氏阳性巨芽孢杆菌对Au NPs的抑菌活性进行了评价。结果显示出浓度依赖性的抗菌性能。最后，对Au NPs在催化和生物医学方面的应用进行了评价。通过4-硝基苯酚转化为4-氨基苯酚的一级动力学，证明了Au NPs的催化活性。使用BMSCs（干细胞）和HeLa（癌细胞）细胞评估细胞摄取和细胞毒性，结果依赖于细胞类型。所合成的金纳米粒子在催化、制药、生物医学等领域具有广阔的应用前景。
This research focuses on the plant-mediated green synthesis process to produce gold nanoparticles (Au NPs) using upland cress (Barbarea verna), as various biomolecules within the upland cress act as both reducing and capping agents. The synthesized gold nanoparticles were thoroughly characterized using UV-vis spectroscopy, surface charge (zeta potential) analysis, scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD). The results indicated the synthesized Au NPs are spherical and well-dispersed with an average diameter ~11 nm and a characteristic absorbance peak at ~529 nm. EDX results showed an 11.13% gold content. Colloidal Au NP stability was confirmed with a zeta potential (ζ) value of −36.8 mV. X-ray diffraction analysis verified the production of crystalline face-centered cubic gold. Moreover, the antimicrobial activity of the Au NPs was evaluated using Gram-negative Escherichia coli and Gram-positive Bacillus megaterium. Results demonstrated concentration-dependent antimicrobial properties. Lastly, applications of the Au NPs in catalysis and biomedicine were evaluated. The catalytic activity of Au NPs was demonstrated through the conversion of 4-nitrophenol to 4-aminophenol which followed first-order kinetics. Cellular uptake and cytotoxicity were evaluated using both BMSCs (stem) and HeLa (cancer) cells and the results were cell type dependent. The synthesized Au NPs show great potential for various applications such as catalysis, pharmaceutics, and biomedicine.
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影响因子：
4.6
作者：
Botteon CEA;Silva LB;Ccana-Ccapatinta GV;Silva TS;Ambrosio SR;Veneziani RCS;Bastos JK;Marcato PD
通讯作者：
Marcato PD
影响因子：
7.4
作者：
Haiss, Wolfgang;Thanh, Nguyen T. K.;Fernig, David G.
通讯作者：
Fernig, David G.
DOI：
10.1515/zpch-2014-0644
发表时间：
2015-01-01
影响因子：
2.5
作者：
O'Neill, Maeve;Raghuwanshi, Vikram Singh;Rademann, Klaus
通讯作者：
Rademann, Klaus
DOI：
10.3390/nano8030174
发表时间：
2018-03-19
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Aljabali AAA;Akkam Y;Al Zoubi MS;Al-Batayneh KM;Al-Trad B;Abo Alrob O;Alkilany AM;Benamara M;Evans DJ
通讯作者：
Evans DJ
DOI：
10.1016/j.saa.2015.01.109
发表时间：
2015-05-05
影响因子：
4.4
作者：
Guo, Mingxia;Li, Wei;Liu, Huihong
通讯作者：
Liu, Huihong