Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities.
Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities.
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DOI：
10.1038/s41598-021-81281-w
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发表时间：
2021-01-21
影响因子：
4.6
通讯作者：
Marcato PD
中科院分区：
文献类型：
--
作者：
Botteon CEA;Silva LB;Ccana-Ccapatinta GV;Silva TS;Ambrosio SR;Veneziani RCS;Bastos JK;Marcato PD
金纳米粒子（AuNPs）由于其低毒性，与人体的相容性，高表面积与体积比以及可以容易地用配体修饰的表面而受到重视。使用植物提取物生物合成AuNPs被认为是一种简单、低成本和生态友好的方法。巴西红蜂胶（BRP）是蜜蜂的产物，具有抗炎、抗肿瘤、抗氧化和抗菌活性。在这里，我们描述了使用BRP提取物（AuNP提取物）及其级分（AuNPhexane，AuNP二氯甲烷，AuNP乙酸乙酯）的AuNP的生物合成，并评估了它们的结构特性及其对微生物和癌细胞的潜力。AuNPs在535 nm处显示出表面等离子体共振（SPR）带。反应中所用的BRP样品的大小和形态受到影响。FTIR和TGA分析表明，BRP提取物或其组分中的生物活性化合物参与了金纳米粒子的合成和稳定。AuNP二氯甲烷和AuNPhexane对所有测试的菌株都表现出抗菌活性，显示了它们作为抗菌剂治疗感染性疾病的功效。AuNPs在T24和PC-3细胞中均表现出剂量依赖性的细胞毒活性。AuNP二氯甲烷和AuNP提取物的体外细胞毒作用最强。此外，生物纳米颗粒的细胞毒性由与细胞凋亡相关的机制诱导。结果突出了一个潜在的低成本的绿色方法，使用巴西红色素合成金纳米粒子，表现出显着的生物学特性。
Gold nanoparticles (AuNPs) are highlighted due to their low toxicity, compatibility with the human body, high surface area to volume ratio, and surfaces that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis (BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl acetate) and evaluated their structural properties and their potential against microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR) band at 535 nm. The sizes and morphologies were influenced by the BRP sample used in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from BRP extract or its fractions in the synthesis and stabilization of AuNPs. AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all strains tested, showing their efficacy as antimicrobial agents to treat infectious diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3 cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms associated with apoptosis. The results highlight a potential low-cost green method using Brazilian red propolis to synthesize AuNPs, which demonstrated significant biological properties.
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影响因子：
3.5
作者：
Araujo, Renara Guedes;Fonseca, Vania de Matos;Ramos, Eloane Goncalves
通讯作者：
Ramos, Eloane Goncalves
影响因子：
4.6
作者：
Azri, Farah Asilah;Selamat, Jinap;Jambari, Nuzul Noorahya
通讯作者：
Jambari, Nuzul Noorahya
影响因子：
2.9
作者：
Barbosa, Valcilaine T.;Souza, Joyelanne K. C.;Dornelas, Camila B.
通讯作者：
Dornelas, Camila B.
影响因子：
5.9
作者：
Cao J;Han J;Xiao H;Qiao J;Han M
通讯作者：
Han M
影响因子：
4.6
作者：
Fierascu I;Georgiev MI;Ortan A;Fierascu RC;Avramescu SM;Ionescu D;Sutan A;Brinzan A;Ditu LM
通讯作者：
Ditu LM