Human umbilical cord blood cells restore brain damage induced changes in rat somatosensory cortex.
Human umbilical cord blood cells restore brain damage induced changes in rat somatosensory cortex.
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DOI：
10.1371/journal.pone.0020194
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Meier C
中科院分区：
文献类型：
--
作者：
Geissler M;Dinse HR;Neuhoff S;Kreikemeier K;Meier C
人脐血(HUCB)细胞腹膜内移植可减少新生大鼠缺氧缺血性脑损伤后的感觉运动障碍。然而，神经功能恢复的相关性以及这种治疗如何在神经处理水平上强制进行可塑性重塑仍然难以捉摸。在这里，我们通过活体记录显示，hUCB细胞有能力改善初级体感皮质中与损伤相关的神经加工障碍。完整的皮质处理依赖于抑制和兴奋传递的微妙平衡，而这种平衡在损伤后会受到干扰。我们发现，损伤后显著改变的大脑皮层地图和感受野的尺寸在很大程度上得到了恢复。此外，在hUCB处理的动物中，不再观察到损伤引起的过度兴奋，这是由类似于在对照动物中观察到的成对脉冲行为所表明的。对皮质加工的有益影响反映在感觉运动行为的几乎完全恢复上。我们的结果表明，人脐血细胞通过正常化抑制和兴奋过程，重新安装了中枢神经元处理信息的方式。我们认为，皮质加工的中间水平将成为研究细胞疗法在脑损伤治疗中的有效性和机制的新阶段。
Intraperitoneal transplantation of human umbilical cord blood (hUCB) cells has been shown to reduce sensorimotor deficits after hypoxic ischemic brain injury in neonatal rats. However, the neuronal correlate of the functional recovery and how such a treatment enforces plastic remodelling at the level of neural processing remains elusive. Here we show by in-vivo recordings that hUCB cells have the capability of ameliorating the injury-related impairment of neural processing in primary somatosensory cortex. Intact cortical processing depends on a delicate balance of inhibitory and excitatory transmission, which is disturbed after injury. We found that the dimensions of cortical maps and receptive fields, which are significantly altered after injury, were largely restored. Additionally, the lesion induced hyperexcitability was no longer observed in hUCB treated animals as indicated by a paired-pulse behaviour resembling that observed in control animals. The beneficial effects on cortical processing were reflected in an almost complete recovery of sensorimotor behaviour. Our results demonstrate that hUCB cells reinstall the way central neurons process information by normalizing inhibitory and excitatory processes. We propose that the intermediate level of cortical processing will become relevant as a new stage to investigate efficacy and mechanisms of cell therapy in the treatment of brain injury.
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兴奋性和抑制性可塑性对突触驱动的神经元输出输出功能的差异影响。
影响因子：
16.2
作者：
Carvalho, Tiago P.;Buonomano, Dean V.
通讯作者：
Buonomano, Dean V.
DOI：
10.1073/pnas.0611723104
发表时间：
2007-03-06
影响因子：
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作者：
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通讯作者：
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影响因子：
25
作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
Hickmott, Peter W.
影响因子：
2.5
作者：
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通讯作者：
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