Blechschmidt Collection: Revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques
Blechschmidt Collection: Revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques
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Blechschmidt Collection：使用现代成像技术重新审视历史上连续切片的人类胚胎和胎儿收藏中的标本
DOI：
10.1111/cga.12261
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发表时间：
2018
影响因子：
1.3
通讯作者：
Yamada Shigehito
中科院分区：
文献类型：
--
作者：
Miyazaki Reina;Makishima Haruyuki;Maenner J;Sydow Hans-Georg;Uwabe Chigako;Takakuwa Tetsuya;Viebahn Christoph;Yamada Shigehito
沿着与美国的卡内基收藏和日本的京都收藏一样，Blechschloven收藏（格奥尔格-奥古斯特-哥廷根大学，德国）是一个重要的人类胚胎和胎儿历史收藏。这些标本对人类胚胎学具有巨大的价值;然而，由于历史组织学标本的性质，一些染色剂的颜色正在褪色，一些载玻片随着时间的推移正在恶化。为了保护这些标本免受这种退化，并确保其未来的有用性，我们试图应用现代图像扫描和计算重建。使用分辨率为4800像素/英寸的商用平板扫描仪将Blechschloven Collection的组织学标本样本数字化为图像。通过使用现代技术将切片的二维图像垂直堆叠成3D块，将两个标本重建成三维（3D）图像。冠臀长（CRL）为64.0 mm的较大标本，是人类胚胎学中一系列非常大的组织学切片，在堆叠前对其中枢神经系统进行了清晰重建。CRL 17.5 mm的较小样本也重建成3D图像。胚胎的外表面是完整的，其发育根据广泛使用的卡内基阶段（CS）进行分类。样品的CS被鉴定为CS 20的后半部分。珍贵的Blechschloven收藏品可以通过现代技术如数字图像扫描和计算3D重建进行进一步研究。
Along with the Carnegie Collection in the United States and the Kyoto Collection in Japan, the Blechschmidt Collection (Georg‐August‐University of Göttingen, Germany) is a major historical human embryo and fetus collection. These collections are of enormous value to human embryology; however, due to the nature of the historical histological specimens, some stains are fading in color, and some glass slides are deteriorating over time. To protect these specimens against such degradation and ensure their future usefulness, we tried to apply modern image scanning and computational reconstruction. Samples of histological specimens of the Blechschmidt Collection were digitized into images using commercial flatbed scanners with a resolution of 4800 pixels per inch. Two specimens were reconstructed into three‐dimensional (3D) images by using modern techniques to vertically stack two‐dimensional images of the slices into 3D blocks. The larger specimen of crown‐rump length (CRL) 64.0 mm, a series of very large histological sections in human embryology, was reconstructed clearly, with its central nervous system segmented before stacking. The smaller specimen of CRL 17.5 mm was also reconstructed into 3D images. The outer surface of the embryo was intact, and its development was classified according to the widely used Carnegie stages (CSs). The CS of the specimen was identified as the later half of CS 20. The invaluable Blechschmidt Collection can be revisited for further research with modern techniques such as digital image scanning and computational 3D reconstruction.
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非洲爪蟾发育图谱
DOI：
10.1007/978-88-470-2905-7
发表时间：
1999
期刊：
--
影响因子：
--
作者：
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DOI：
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发表时间：
1979
期刊：
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DOI：
10.30965/9783846756966_004
发表时间：
2014
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影响因子：
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10.1002/bies.950060213
发表时间：
1987
期刊：
BioEssays : news and reviews in molecular, cellular and developmental biology
影响因子：
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2005
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