Strategies to potentiate antimicrobial photoinactivation by overcoming resistant phenotypes.
Strategies to potentiate antimicrobial photoinactivation by overcoming resistant phenotypes.
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DOI：
10.1111/j.1751-1097.2012.01087.x
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发表时间：
2012-05
影响因子：
3.3
通讯作者：
Tegos GP
中科院分区：
文献类型：
--
作者：
Vera DM;Haynes MH;Ball AR;Dai T;Astrakas C;Kelso MJ;Hamblin MR;Tegos GP
由于病原微生物多重耐药性的出现，传统的抗菌策略已变得越来越无效。克服这些缺陷的需要引发了对控制微生物感染的替代治疗和非常规方法的探索。光动力学疗法最初被确立为一种抗癌方式，目前用于治疗年龄相关性黄斑变性。光动力失活的概念需要细胞暴露于光能，通常是可见光区的波长，其引起光敏剂分子的激发，无论是外源性的还是内源性的，这导致活性氧物质的产生。活性氧通过修饰细胞内组分而使细胞失活和死亡。PDT的多功能特性促使其作为抗感染发现平台进行研究。对微生物生理学理解的进展揭示了一系列途径和表型，这些途径和表型可作为抗菌药物发现的假定靶点。据报道，对这些表型元件与PDT的研究重点是多药外排系统、生物膜、毒力和发病机制决定因素。在许多情况下，结果是有希望的，但只是初步的，需要进一步调查。这篇综述讨论了不同的抗微生物PDT策略，并强调需要高度信息化和全面的发现方法。
Conventional antimicrobial strategies have become increasingly ineffective due to the emergence of multidrug resistance among pathogenic microorganisms. The need to overcome these deficiencies has triggered the exploration of alternative treatments and unconventional approaches towards controlling microbial infections. Photodynamic therapy was originally established as an anti-cancer modality and is currently used in the treatment of age related macular degeneration. The concept of photodynamic inactivation requires cell exposure to light energy, typically wavelengths in the visible region that causes the excitation of photosensitizer molecules either exogenous or endogenous, which results in the production of reactive oxygen species. ROS produce cell inactivation and death through modification of intracellular components. The versatile characteristics of PDT prompted its investigation as an anti-infective discovery platform. Advances in understanding of microbial physiology have shed light on a series of pathways, and phenotypes that serve as putative targets for antimicrobial drug discovery. Investigations of these phenotypic elements in concert with PDT have been reported focused on multidrug efflux systems, biofilms, virulence and pathogenesis determinants. In many instances the results are promising but only preliminary and require further investigation. This review discusses the different antimicrobial PDT strategies and highlights the need for highly informative and comprehensive discovery approaches.
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DOI：
10.1007/978-1-60761-697-9_13
发表时间：
2010-01-01
期刊：
PHOTODYNAMIC THERAPY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Biel, Merrill A.
通讯作者：
Biel, Merrill A.
影响因子：
4
作者：
Ball, Anthony R.;Casadei, Gabriele;Lewis, Kim
通讯作者：
Lewis, Kim
影响因子：
3.1
作者：
Bisland, SK;Chien, C;Burch, S
通讯作者：
Burch, S
影响因子：
3.7
作者：
Dutta NK;Mehra S;Kaushal D
通讯作者：
Kaushal D
影响因子：
6.7
作者：
del Pozo, J. L.;Patel, R.
通讯作者：
Patel, R.