The peptide GOLVEN10 controls nodule and lateral root organogenesis and positioning along the longitudinal root axis
The peptide GOLVEN10 controls nodule and lateral root organogenesis and positioning along the longitudinal root axis
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肽 GOLVEN10 控制根瘤和侧根器官发生以及沿纵根轴的定位
DOI：
10.1101/2022.05.06.490929
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Roy S
中科院分区：
文献类型：
--
作者：
Roy S
在所有能够形成根或类根结构的植物的基因组中都可以检测到GLV/RGF多肽编码基因，这表明它们是植物向陆地过渡所必需的。在紫花苜蓿中，15个GOLVEN(GLV)/根分生组织生长因子(RGF)多肽编码基因中的5个在根瘤器官发生过程中以及在缺氮和生长素处理下不同程度地诱导表达。MtGLV9和MtGLV10在结瘤起始部位的表达与转录因子结瘤相关。结瘤诱导的所有5个GLV基因在结瘤诱导下均过表达。毛状根和相应的合成肽的应用使根瘤数量减少了25%-50%，说明GOLVEN是根瘤器官发生的负调节因子。GOLVEN10肽使第一个形成的侧根(趋根性)和第一个形成的根瘤沿纵向主根轴移动，这种现象我们称为趋根性，从而缩短了根上侧器形成区的绝对长度。应用合成的GOLVEN10肽使每个根皮质细胞层的细胞数量增加，但不是细胞长度增加，导致根长度增加，从而导致第一侧器形成的时空延迟。简言之，节位是正在研究的一个特征然而，它决定了能够支持根瘤形成的根的长度，从而决定了形成的功能根瘤的总数。我们首次发现了被称为GOLVEN肽的遗传因素，它改变了根瘤和侧根在初生根上的位置，以及包括根瘤器官起始和根构型在内的其他几个特征。
GLV/RGF peptide encoding genes can be identified in genomes of all plants that can form roots or root-like structures suggesting they were essential for transition of plants to land.InMedicago truncatula, five of fifteen GOLVEN(GLV)/ROOT MERISTEM GROWTH FACTOR (RGF) peptide coding genes were induced during nodule organogenesis and to a varying extent under nitrogen deficiency and auxin treatment. Expression ofMtGLV9andMtGLV10at nodule initiation sites was dependent on the transcription factor NODULE INCEPTION.Overexpression of all five nodule-inducedGLVgenes inM. truncatulahairy roots as well as application of the corresponding synthetic peptides resulted in a 25-50% reduction in nodule number indicating GOLVENs are negative regulators of nodule organogenesis.The peptide GOLVEN10 shifted the position of the first formed lateral root (rhizotaxis) as well as the first formed nodule along the longitudinal primary root axis, a phenomenon we term ‘nodulotaxis’, thereby reducing the absolute length of the zone of lateral organ formation on roots.Application of synthetic GOLVEN10 peptide caused an increase in cell number but not cell length in each root cortical cell layer causing an increase in root length and a consequent spatiotemporal delay in formation of the first lateral organ.Plain Language SummaryNodule positioning is an understudied trait, yet it determines the length of the root that can support nodule formation and consequently the total number of functional nodules formed. We identify for the first time, genetic factors called GOLVEN peptides that alter nodule and lateral root positioning on the primary root along with several other traits including nodule organ initiation and root architecture.
影响因子：
4.9
作者：
Goto H;Okuda S;Mizukami A;Mori H;Sasaki N;Kurihara D;Higashiyama T
通讯作者：
Higashiyama T
DOI：
10.1111/nph.13405
发表时间：
2015-08
期刊：
The New phytologist
影响因子：
--
作者：
Cederholm HM;Benfey PN
通讯作者：
Benfey PN