Molecular evidence for the thriving of Campylobacter jejuni ST-4526 in Japan.
Molecular evidence for the thriving of Campylobacter jejuni ST-4526 in Japan.
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DOI：
10.1371/journal.pone.0048394
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Igimi S
中科院分区：
文献类型：
--
作者：
Asakura H;Brüggemann H;Sheppard SK;Ekawa T;Meyer TF;Yamamoto S;Igimi S
空肠弯曲杆菌是世界范围内人类肠胃炎的主要原因。本研究旨在更好地了解这种在日本传播的病原体的遗传多样性。2005年至2006年，我们对日本不同来源的空肠弯曲杆菌（100株人、61株家禽和51株牛）进行了多位点序列分型（MLST）。该方法鉴定出62个序列类型（STs）和19个克隆复合物（CCs），其中包括11个新的STs。这62个STs在系统发育上被划分为6个簇，部分显示出宿主关联。我们发现了一种在其他国家从未报道过的新型ST (ST-4526)；系统发育分析表明，ST-4526及其相关的ST与CC-21的始祖ST-21有较远的亲缘关系。进行了比较基因组分析，以调查哪些特性可能导致ST-4526在日本成功传播。结果表明，3株具有代表性的ST-4526分离株与参考菌株NCTC11168 （ST-43/CC-21）存在不同的推定岛，该推定岛位于Cj0737 ~ Cj0744区域。氨基酸序列比对分析表明，3株ST-4526菌株中有2株表达693aa-丝状血凝域蛋白（FHA），而其他大部分菌株的基因组测序结果显示其缺失。相应的，fha阳性的空肠梭菌与宿主细胞的结合程度大于fha截断的菌株，外源给予rFHA蛋白降低了fha阳性菌的细胞粘附。生化分析表明，这种假定的蛋白对硫酸肝素具有剂量依赖性的结合亲和力，表明其具有粘附活性。此外，ST-4526对抗生素（萘啶酸和氟喹诺酮类药物）的耐药性增强，DNA摄取能力降低。综上所述，我们的数据表明，这些综合特征促成了ST-4526在日本的克隆繁荣。
Campylobacter jejuni is a leading cause of human gastroenteritis worldwide. This study aimed at a better understanding of the genetic diversity of this pathogen disseminated in Japan. We performed multilocus sequence typing (MLST) of Campylobacter jejuni isolated from different sources (100 human, 61 poultry, and 51 cattle isolates) in Japan between 2005 and 2006. This approach identified 62 sequence types (STs) and 19 clonal complexes (CCs), including 11 novel STs. These 62 STs were phylogenetically divided into 6 clusters, partially exhibiting host association. We identified a novel ST (ST-4526) that has never been reported in other countries; a phylogenetic analysis showed that ST-4526 and related STs showed distant lineage from the founder ST, ST-21 within CC-21. Comparative genome analysis was performed to investigate which properties could be responsible for the successful dissemination of ST-4526 in Japan. Results revealed that three representative ST-4526 isolates contained a putative island comprising the region from Cj0737 to Cj0744, which differed between the ST-4526 isolates and the reference strain NCTC11168 (ST-43/CC-21). Amino acid sequence alignment analyses showed that two of three ST-4526 isolates expressed 693aa- filamentous hemagglutination domain protein (FHA), while most of other C. jejuni strains whose genome were sequenced exhibited its truncation. Correspondingly, host cell binding of FHA-positive C. jejuni was greater than that of FHA-truncated strains, and exogenous administration of rFHA protein reduced cell adhesion of FHA-positive bacteria. Biochemical assays showed that this putative protein exhibited a dose-dependent binding affinity to heparan sulfate, indicating its adhesin activity. Moreover, ST-4526 showed increased antibiotic-resistance (nalidixic acid and fluoroquinolones) and a reduced ability for DNA uptake. Taken together, our data suggested that these combined features contributed to the clonal thriving of ST-4526 in Japan.
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