Dorsoventral inversion of the air-filled organ (lungs, gas bladder) in vertebrates: RNAsequencing of laser capture microdissected embryonic tissue.
Dorsoventral inversion of the air-filled organ (lungs, gas bladder) in vertebrates: RNAsequencing of laser capture microdissected embryonic tissue.
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DOI：
10.1002/jez.b.22998
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
McCune A
中科院分区：
文献类型：
--
作者：
Funk E;Lencer E;McCune A
基因表达的改变如何产生新的性状是理解进化过程的关键。我们研究了鱼类气囊起源于祖先骨脊椎动物肺的遗传基础。区别这些同源器官是在发育过程中从前肠出芽的方向;肺在腹侧出芽，气囊在背侧出芽。我们研究了这种形态学倒位是否与调节肺和膀胱发育的保守基因的分子倒位有关。使用激光捕获显微切割和RNA-seq，我们测定了转录丰度和比较背腹前肠组织在三个发育阶段跨越gasbladder发展的表达模式。我们的焦点分类群，bowfin（Amia calva），代表硬骨鱼的姐妹群，是一个早期的分歧射线鳍鱼与气膀胱。我们发现了一些在肺发育过程中具有未知功能的基因，这些基因在气囊发育过程中差异表达，并被注释为与器官出芽相关的功能。我们还确定了几个已知的肺调节基因表现出反向背腹表达在gasbladder相对于肺的发展。具体来说，我们发现Tbx 5强烈表达在背鳍发育过程中的气囊周围的背侧中胚层，和几个相互作用的基因与Tbx 5背侧共表达。相比之下，在小鼠和bichir（Polypterus senegalus），唯一的射线鳍鱼，有肺，Tbx 5在腹侧肺中胚层发育过程中表达。我们的数据表明保守基因的背腹反转表明，这些基因可能有助于腹侧肺和背侧气膀胱的射线鳍鱼之间的进化过渡。
How modification of gene expression generates novel traits is key to understanding the evolutionary process. We investigated the genetic basis for the origin of the piscine gas bladder from lungs of ancestral bony vertebrates. Distinguishing these homologous organs is the direction of budding from the foregut during development; lungs bud ventrally and the gas bladder buds dorsally. We investigated whether this morphological inversion is associated with molecular inversion of conserved genes regulating lung and gasbladder development. Using laser-capture microdissection and RNA-seq, we assayed transcript abundance and compared expression patterns between dorsal and ventral foregut tissues at three developmental stages spanning gasbladder development. Our focal taxon, bowfin (Amia calva), representing the sistergroup to teleosts, is an early diverging ray-finned fish with a gas bladder. We discovered a number of genes with unknown function during lung development that are differentially expressed during gas bladder development and annotated to functions relevant for organ budding. We also identified several known lung-regulatory genes exhibiting inverted dorsoventral expression during gasbladder relative to lung development. Specifically, we found Tbx5 is strongly expressed in the dorsal mesoderm surrounding the gas bladder during bowfin development, and several interacting genes are co-expressed dorsally with Tbx5. In contrast, in mouse and bichir (Polypterus senegalus), the only ray-finned fish that have lungs, Tbx5 is expressed in the ventral lung mesoderm during development. Our data demonstrating dorsoventral inversion of conserved genes suggests that these genes may have contributed to the evolutionary transition between ventral lungs and a dorsal gas bladder in ray-finned fishes.
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影响因子：
3.4
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