Epstein‐Barr virus expression within keratinizing nasopharyngeal carcinoma
Epstein‐Barr virus expression within keratinizing nasopharyngeal carcinoma
复制标题
EB病毒在角化鼻咽癌中的表达
作者：
J. X. Zhang;H. L. Chen;Y. Zong;K. Chan;J. Nicholls;Jaap M Middeldorp;J. Sham;B. Griffin;M. Ng
在角化型鼻咽癌中可以看到三个成熟阶段。这些阶段在形态上类似于正常鳞状上皮中的基底细胞、中间和表面鳞状细胞。利用这种多样化的肿瘤细胞群，通过使用EBV编码的RNA（EBER）的原位杂交检测10例角化性鼻咽癌（NPC）是否存在潜伏性EB-巴尔病毒（EBV），并通过免疫组织学检测是否存在EBV早期抗原扩散（EA-D）和EBV的350/220 kd膜糖蛋白。基底细胞样肿瘤细胞主要被病毒潜伏感染;在分离的中间鳞状细胞中发现病毒复制，而浅表鳞状细胞基本上耗尽了所有病毒标志物。我们使用了由未分化和低分化肿瘤细胞组成的非角化鼻咽癌对照系列，这些肿瘤中存在EBV潜伏期。在未分化肿瘤中通过RT-PCR检测到病毒复制，但通过免疫组织学未观察到病毒复制。根据这些发现，在这些肿瘤和肿瘤细胞分化的EBV的生命周期之间的可能关系进行了讨论。J. Med. Virol. 55：227-233，1998.© 1998 Wiley利斯公司
Three stages of maturation can be seen in keratinizing nasopharyngeal carcinomas. These stages are similar morphologically to basal cells, intermediate and superficial squamous cells seen in normal squamous epithelium. Taking advantage of such a diverse tumour cell population, 10 keratinizing nasopharyngeal carcinoma (NPC) were examined by in situ hybridization for the presence of latent Epstein‐Barr Virus (EBV) using EBV encoded RNAs (EBERs) and by immunohistology for the presence of EBV early antigen‐diffuse (EA‐D) and the 350/220 kd membrane glycoprotein of the EBV. The basal cell‐like tumour cells are mainly infected latently with the virus; viral replication was found in isolated intermediate squamous cells, whilst superficial squamous cells are largely depleted of all the viral markers. We used a control series of non‐keratinizing nasopharyngeal carcinomas composed of undifferentiated and poorly differentiated tumour cells and EBV latency was present in these tumours. Viral replication was detected by RT‐PCR, in the undifferentiated tumours but viral replication was not seen by immunohistology. The possible relationship between EBV life cycle in these tumours and tumour cell differentiation is discussed in the light of these findings. J. Med. Virol. 55:227–233, 1998. © 1998 Wiley‐Liss, Inc.
影响因子：
3.7
作者：
LUNG, ML;CHANG, RS;NG, MH
通讯作者：
NG, MH
DOI：
10.1073/pnas.77.9.5163
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DATTA, AK;COLBY, BM;PAGANO, JS
通讯作者：
PAGANO, JS
Epstein-Barr病毒在口咽上皮细胞中的复制。
DOI：
10.1056/nejm198405103101905
发表时间：
1984
期刊：
The New England journal of medicine
影响因子：
--
作者：
Sixbey,JW;Nedrud,JG;Raab-Traub,N;Hanes,RA;Pagano,JS
通讯作者：
Pagano,JS