Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. I. Lung epithelial morphogenesis.-猫眼课题宝

E-和P-钙粘蛋白粘附分子在胚胎组织发生中的表达和作用。

DOI：

发表时间：

1989

期刊：

Development

影响因子：

4.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Y. Hirai;A. Nose;S. Kobayashi;M. Takeichi

研究了 Ca2+ 依赖性细胞间粘附分子 E- 和 P-钙粘蛋白在小鼠胚胎肺组织发生中的作用。所有肺上皮细胞在早期发育阶段均表达 E-钙粘蛋白和 P-钙粘蛋白。然而，P-钙粘蛋白在发育过程中逐渐消失，最初从主支气管消失，最终从所有上皮细胞消失。当将 E-钙粘蛋白 (ECCD-1) 单克隆抗体添加到肺上皮细胞的单层培养物中时，会诱导细胞间粘附的部分破坏，而 P-钙粘蛋白 (PCD-1) 单克隆抗体则显示出微妙的效果。然而，两种抗体的混合物显示出协同效应。然后，我们使用器官培养系统测试了抗体对肺原基形态发生的影响。在对照培养基中，外植体形成典型的支气管树。在 ECCD-1 存在的情况下，外植体的生长速度与对照相同，但其形态发生受到影响。对照外植体形成圆形上皮小叶，在支气管树的尖端处具有开放的腔空间，而用ECCD-1孵育的外植体的小叶往往是平坦的并且没有腔空间。 PCD-1 显示出类似但非常小的效果。然而，两种抗体的混合物显示出更强的效果：上皮细胞的分支被部分抑制，并且上皮细胞的排列在许多地方被扭曲。这些结果表明E-钙粘蛋白和P-钙粘蛋白在肺形态发生中的上皮细胞组织中具有协同作用。

The role of Ca2+-dependent cell-cell adhesion molecules, E- and P-cadherins, in the histogenesis of mouse embryonic lung was studied. All epithelial cells of the lung express both E- and P-cadherin at the early developmental stage. P-cadherin, however, gradually disappears during development, initially from the main bronchi and eventually from all epithelial cells. When a monoclonal antibody to E-cadherin (ECCD-1) was added to monolayer cultures of lung epithelial cells, it induced a partial disruption of their cell-cell adhesion, while a monoclonal antibody to P-cadherin (PCD-1) showed a subtle effect. A mixture of the two antibodies, however, displayed a synergistic effect. We then tested the effect of the antibodies on the morphogenesis of lung primordia using an organ culture system. In control media, the explants formed typical bronchial trees. In the presence of ECCD-1, the explants grew up at the same rate as in the control, but their morphogenesis was affected. The control explants formed round epithelial lobules with an open luminal space at the tips of the bronchial trees, whereas the lobules of explants incubated with ECCD-1 tended to be flat and devoid of the luminal space. PCD-1 showed a similar but very small effect. A mixture of the two antibodies, however, showed a stronger effect: the branching of epithelia was partially suppressed and the arrangement of epithelial cells was distorted in many places. These results suggest that E- and P-cadherin have a synergistic role in the organization of epithelial cells in lung morphogenesis.

Antibodies to liver cell adhesion molecule perturb inductive interactions and alter feather pattern and structure.