Investigation on absorption cross-section of photosynthetic pigment molecules based on a mechanistic model of the photosynthetic electron flow-light response in C(3), C(4) species and cyanobacteria grown under various conditions.
Investigation on absorption cross-section of photosynthetic pigment molecules based on a mechanistic model of the photosynthetic electron flow-light response in C(3), C(4) species and cyanobacteria grown under various conditions.
复制标题
DOI：
10.3389/fpls.2023.1234462
复制
发表时间：
2023
影响因子：
5.6
通讯作者：
Wang, Fu-Biao
中科院分区：
文献类型：
--
作者：
Ye, Zi-Piao;Stirbet, Alexandrina;An, Ting;Robakowski, Piotr;Kang, Hua-Jing;Yang, Xiao-Long;Wang, Fu-Biao
关键词：
研究参与太阳能吸收和激发的光合色素分子的内在性质，特别是其本征吸收截面（σ ik）和有效吸收截面（σ ′ ik），对于理解光合作用具有重要意义。在这里，我们提出了一种新的方法来确定这些参数的开发和应用，基于光合电子流光响应的机械模型。用我们的方法对一系列先前收集的叶绿素a荧光数据的分析表明，对于在各种条件下生长的几种光合生物，光合色素分子的吸收截面具有大约10 - 21 m2的不同值：（1）针叶树冷杉，在强光或弱光下生长;（2）红豆杉（TaxusbaccataL.），（3）大豆（Glycine max L.）（Merr.），在遮荫条件下在叶室中在400或600 μmol CO2 mol-1的CO2浓度下生长;（4）玉米L.，在30°C或35°C的温度下在叶室中培养;（5）桂花，具有遮荫叶或阳光叶;和（6）蓝细菌铜绿微囊藻FACHB 905，在两种不同的氮供应下生长。我们的研究结果表明，σ ik具有相同的数量级（约10−21 m2），这些物种的σ ′ ik随着光照强度的增加而降低，这表明了一种关键的调节机制的运作，以减少太阳能吸收并避免强光损伤。此外，与其他方法相比，我们的方法可以更容易地估计σ ik和σ ′ ik，即使在各种生长条件下（例如，不同的光环境;不同的CO2、NO2、O2和O3浓度;空气温度;或水压力），而不管样品的类型（例如，稀释或浓缩的细胞悬浮液或叶）。结果还表明，CO2浓度和温度对G. max和Z.梅斯因此，我们的方法提供了一个强大的工具来研究光合色素分子的光能吸收，并为我们提供了新的信息，植物和蓝藻如何在不同的胁迫条件下修改其捕光性能。
Investigation on intrinsic properties of photosynthetic pigment molecules participating in solar energy absorption and excitation, especially their eigen-absorption cross-section (σ ik) and effective absorption cross-section (σ ′ ik), is important to understand photosynthesis. Here, we present the development and application of a new method to determine these parameters, based on a mechanistic model of the photosynthetic electron flow-light response. The analysis with our method of a series of previously collected chlorophyll a fluorescence data shows that the absorption cross-section of photosynthetic pigment molecules has different values of approximately 10−21 m2, for several photosynthetic organisms grown under various conditions: (1) the conifer Abies alba Mill., grown under high light or low light; (2) Taxus baccata L., grown under fertilization or non-fertilization conditions; (3) Glycine max L. (Merr.), grown under a CO2 concentration of 400 or 600 μmol CO2 mol−1 in a leaf chamber under shaded conditions; (4) Zea mays L., at temperatures of 30°C or 35°C in a leaf chamber; (5) Osmanthus fragrans Loureiro, with shaded-leaf or sun-leaf; and (6) the cyanobacterium Microcystis aeruginosa FACHB905, grown under two different nitrogen supplies. Our results show that σ ik has the same order of magnitude (approximately 10−21 m2), and σ ′ ik for these species decreases with increasing light intensity, demonstrating the operation of a key regulatory mechanism to reduce solar absorption and avoid high light damage. Moreover, compared with other approaches, both σ ik and σ ′ ik can be more easily estimated by our method, even under various growth conditions (e.g., different light environment; different CO2, NO2, O2, and O3 concentrations; air temperatures; or water stress), regardless of the type of the sample (e.g., dilute or concentrated cell suspensions or leaves). Our results also show that CO2 concentration and temperature have little effect on σ ik values for G. max and Z. mays. Consequently, our approach provides a powerful tool to investigate light energy absorption of photosynthetic pigment molecules and gives us new information on how plants and cyanobacteria modify their light-harvesting properties under different stress conditions.
登录
查看更多内容
影响因子：
16.6
作者：
Grayson KJ;Faries KM;Huang X;Qian P;Dilbeck P;Martin EC;Hitchcock A;Vasilev C;Yuen JM;Niedzwiedzki DM;Leggett GJ;Holten D;Kirmaier C;Neil Hunter C
通讯作者：
Neil Hunter C
DOI：
10.1016/s0005-2728(05)80062-3
发表时间：
1991-02-08
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BIXON, M;JORTNER, J;MICHELBEYERLE, ME
通讯作者：
MICHELBEYERLE, ME
影响因子：
3.7
作者：
GOVINDJEE
通讯作者：
GOVINDJEE
COMT1 沉默通过降低番茄中叶绿素含量、光系统 II 活性和电子传输效率来加剧热应激引起的光合作用减少
影响因子：
5.6
作者：
Ahammed GJ;Xu W;Liu A;Chen S
通讯作者：
Chen S
影响因子：
9.4
作者：
Buckley, Thomas N.;Diaz-Espejo, Antonio
通讯作者：
Diaz-Espejo, Antonio