Phase contrast time-lapse microscopy datasets with automated and manual cell tracking annotations.
Phase contrast time-lapse microscopy datasets with automated and manual cell tracking annotations.
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DOI：
10.1038/sdata.2018.237
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发表时间：
2018-11-13
期刊：
影响因子：
9.8
通讯作者：
Campbell PG
中科院分区：
文献类型：
--
作者：
Ker DFE;Eom S;Sanami S;Bise R;Pascale C;Yin Z;Huh SI;Osuna-Highley E;Junkers SN;Helfrich CJ;Liang PY;Pan J;Jeong S;Kang SS;Liu J;Nicholson R;Sandbothe MF;Van PT;Liu A;Chen M;Kanade T;Weiss LE;Campbell PG
Phase contrast time-lapse microscopy is a non-destructive technique that generates large volumes of image-based information to quantify the behaviour of individual cells or cell populations. To guide the development of algorithms for computer-aided cell tracking and analysis, 48 time-lapse image sequences, each spanning approximately 3.5 days, were generated with accompanying ground truths for C2C12 myoblast cells cultured under 4 different media conditions, including with fibroblast growth factor 2 (FGF2), bone morphogenetic protein 2 (BMP2), FGF2 + BMP2, and control (no growth factor). The ground truths generated contain information for tracking at least 3 parent cells and their descendants within these datasets and were validated using a two-tier system of manual curation. This comprehensive, validated dataset will be useful in advancing the development of computer-aided cell tracking algorithms and function as a benchmark, providing an invaluable opportunity to deepen our understanding of individual and population-based cell dynamics for biomedical research.
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DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM
影响因子：
10.6
作者：
Huh, Seungil;Ker, Dai Fei Elmer;Kanade, Takeo
通讯作者：
Kanade, Takeo
影响因子：
48
作者：
Schneider CA;Rasband WS;Eliceiri KW
通讯作者：
Eliceiri KW
CCR7配体刺激体内T淋巴细胞的鼻内运动性。
影响因子：
15.3
作者：
Worbs, Tim;Mempel, Thorsten R.;Boelter, Jasmin;von Andrian, Ulrich H.;Foerster, Reinhold
通讯作者：
Foerster, Reinhold
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert