Condensins promote chromosome recoiling during early anaphase to complete sister chromatid separation.
Condensins promote chromosome recoiling during early anaphase to complete sister chromatid separation.
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DOI：
10.1016/j.devcel.2010.07.013
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发表时间：
2010-08-17
影响因子：
11.8
通讯作者：
Tanaka, Tomoyuki U.
中科院分区：
文献类型：
--
作者：
Renshaw, Matthew J.;Ward, Jonathan J.;Kanemaki, Masato;Natsume, Kayo;Nedelec, Francois J.;Tanaka, Tomoyuki U.
姐妹染色单体的分离是在分裂后期开始时通过分离酶的激活而开始的，分离酶从染色体上去除粘着蛋白。然而，姐妹染色单体的分离是如何沿着沿着整个染色体长度完成的，这仍然是一个谜。在这里，我们发现，在后期早期的酿酒酵母，姐妹染色单体分离逐渐从着丝粒端粒，伴随着区域染色体拉伸和随后的反冲。这种拉伸是由于姐妹染色单体之间残留的凝聚力，这阻止了它们立即分离。这种残余的凝聚力至少部分依赖于在后期开始时逃脱分离酶去除的凝聚素。同时，拉伸的染色体区域的反冲需要凝聚素并产生力来去除残余的凝聚力。我们提供的证据表明，凝聚素促进染色体反冲直接在体内，这是不同的，从他们的已知功能，解决姐妹染色单体。我们的工作确定了在后期早期残留的姐妹染色单体凝聚力，并揭示了凝聚素在染色体反冲中的作用，从而消除了残留的凝聚力，完成姐妹染色单体的分离。在芽殖酵母中，姐妹染色单体的凝聚力在后期开始后部分持续存在。残余凝聚力部分依赖于凝聚素，并导致染色体拉伸。凝聚素直接促进后期早期拉伸染色体的反冲。染色体反冲产生力以去除残余的姐妹染色单体凝聚力。
Sister chromatid separation is initiated at anaphase onset by the activation of separase, which removes cohesins from chromosomes. However, it remains elusive how sister chromatid separation is completed along the entire chromosome length. Here we found that, during early anaphase in Saccharomyces cerevisiae, sister chromatids separate gradually from centromeres to telomeres, accompanied by regional chromosome stretching and subsequent recoiling. The stretching results from residual cohesion between sister chromatids, which prevents their immediate separation. This residual cohesion is at least partly dependent on cohesins that have escaped removal by separase at anaphase onset. Meanwhile, recoiling of a stretched chromosome region requires condensins and generates forces to remove residual cohesion. We provide evidence that condensins promote chromosome recoiling directly in vivo, which is distinct from their known function in resolving sister chromatids. Our work identifies residual sister chromatid cohesion during early anaphase and reveals condensins' roles in chromosome recoiling, which eliminates residual cohesion to complete sister chromatid separation. ► Sister chromatid cohesion partially persists after anaphase onset in budding yeast ► Residual cohesion is partly dependent on cohesins and causes chromosome stretching ► Condensins directly promote recoiling of stretched chromosomes during early anaphase ► Chromosome recoiling generates forces to remove residual sister chromatid cohesion
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影响因子：
9.2
作者：
Paliulis, LV;Nicklas, RB
通讯作者：
Nicklas, RB
DOI：
10.1083/jcb.149.4.811
发表时间：
2000-05-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Freeman L;Aragon-Alcaide L;Strunnikov A
通讯作者：
Strunnikov A
影响因子：
1.6
作者：
Harrison BD;Hoang ML;Bloom K
通讯作者：
Bloom K
影响因子：
64.5
作者：
Gruber, Stephan;Errington, Jeff
通讯作者：
Errington, Jeff
影响因子：
64.8
作者：
Haering, Christian H.;Farcas, Ana-Maria;Nasmyth, Kim
通讯作者：
Nasmyth, Kim