TCTP Expression After Rat Spinal Cord Injury: Implications for Astrocyte Proliferation and Migration
TCTP Expression After Rat Spinal Cord Injury: Implications for Astrocyte Proliferation and Migration
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大鼠脊髓损伤后 TCTP 表达：对星形胶质细胞增殖和迁移的影响
DOI：
10.1007/s12031-015-0628-0
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Ren J;Mao X;Chen M;Zhang W;Liu Y;Duan C;Zhang H;Sun C;Wu W;Zhu X;Ge J;Tao W;Wang Y;Lu H
翻译控制肿瘤蛋白（TCTP）是一种普遍存在且高度保守的蛋白质，在细胞增殖和生长、细胞凋亡和细胞周期调节中发挥作用。然而，其在脊髓损伤（SCI）中的表达和功能仍不清楚。在这里，我们证明了急性脊髓损伤后 TCTP 的表达发生动态变化。我们的结果显示，TCTP 逐渐增加，在脊髓损伤后 3 天达到峰值，然后在脊髓损伤后 14 天下降到基础水平。 TCTP 的上调伴随着 PCNA 等增殖蛋白水平的增加。免疫荧光标记还显示 TCTP 位于星形胶质细胞中，并且创伤性 SCI 诱导 TCTP 与 PCNA 共定位。这些结果表明TCTP可能在星形胶质细胞增殖中发挥重要作用。为了进一步探讨 TCTP 的作用，TCTP 特异性 siRNA 转染的星形胶质细胞显示原代星形胶质细胞增殖显着减少。令人惊讶的是，TCTP 敲除也减少了原代星形胶质细胞的迁移，因为 siRNA 转染后微管和 F-肌动蛋白的重组受到干扰。以上表明TCTP可能在星形胶质细胞增殖和迁移中发挥重要作用。总的来说，我们的数据表明 TCTP 可能在 SCI 后的 CNS 病理生理学中发挥重要作用。
Translationally controlled tumor protein (TCTP) is a ubiquitous and highly conserved protein which plays a role in cell proliferation and growth, apoptosis, and cell cycle regulation. However, its expression and function in spinal cord injury (SCI) are still unknown. Here, we demonstrated that expression of TCTP was dynamic changed after acute spinal cord injury. Our results showed that TCTP gradually increased, reached a peak at 3 day, and then declined to basal levels at 14 days after spinal cord injury. Upregulation of TCTP was accompanied with an increase in the levels of proliferation proteins such as PCNA. Immunofluorescent labeling also showed that TCTP located in astrocytes and traumatic SCI induced TCTP colocalizated with PCNA. These results indicated that TCTP might play an important role in astrocyte proliferation. To further probe the role of TCTP, TCTP-specific siRNA-transfected astrocytes showed significant decrease of primary astrocyte proliferation. Surprisingly, TCTP knockdown also reduced primary astrocyte migration, as the reorganization of microtubules and F-actin was disturbed after siRNA transfection. All above indicated that TCTP might play a crucial role in astrocyte proliferation and migration. Collectively, our data suggested that TCTP might play important roles in CNS pathophysiology after SCI.
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DOI：
10.1080/09638280110110640
发表时间：
2002-01
期刊：
The Lancet
影响因子：
--
作者：
J. Mcdonald;C. Sadowsky
通讯作者：
J. Mcdonald;C. Sadowsky
TRPV1阳离子通道的激活有助于压力诱导的星形胶质细胞迁移。
影响因子：
6.2
作者：
Ho, Karen W.;Lambert, Wendi S.;Calkins, David J.
通讯作者：
Calkins, David J.
DOI：
10.1016/b978-0-12-818422-6.00060-5
发表时间：
2020
期刊：
Principles of Tissue Engineering
影响因子：
--
作者：
N. Madigan;A. Windebank
通讯作者：
N. Madigan;A. Windebank
影响因子：
5.2
作者：
Gnanasekar, Munirathinam;Thirugnanam, Sivasakthivel;Ramaswamy, Kalyanasundaram
通讯作者：
Ramaswamy, Kalyanasundaram
DOI：
10.3171/2011.3.spine11153
发表时间：
2011-11
期刊：
Journal of neurosurgery. Spine
影响因子：
--
作者：
S. Lalwani;P. Mathur;Neetu J. Jain;B. Behera;M. Misra
通讯作者：
S. Lalwani;P. Mathur;Neetu J. Jain;B. Behera;M. Misra