MicroRNA-206 suppresses proliferation and predicts poor prognosis of HR-HPV-positive cervical cancer cells by targeting G6PD.
MicroRNA-206 suppresses proliferation and predicts poor prognosis of HR-HPV-positive cervical cancer cells by targeting G6PD.
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DOI：
10.3892/ol.2018.9326
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发表时间：
2018-11
期刊：
影响因子：
2.9
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Cui J;Pan Y;Wang J;Liu Y;Wang H;Li H
宫颈癌是世界范围内最常见的妇科恶性肿瘤之一。据报道，microRNA(MiR)-206的异常表达在肿瘤进展中起重要作用。本研究的目的是评估miR-206的潜在作用，并验证其对CC中葡萄糖-6-磷酸脱氢酶(G6PD)功能的影响。Western印迹分析和RT-PCR检测miR-206和G6PD的表达。用荧光素酶实验验证G6PD是否为miR-206靶点。CCK-8法检测miR-206和G6PD对CC增殖的调节作用。结果表明，高危型人乳头瘤病毒(HR-HPV)(+)CC中miR-206表达下调，而G6PD表达上调。MIR-206直接针对G6PD的3‘-非编码区。MIR-206过表达或G6PD低表达抑制细胞增殖。MIR-206低表达或G6PD高表达预示预后不良。总之，miR-206抑制了CC中肿瘤的生长，并抑制了G6PD的表达。这一新发现的miR-206可能为肿瘤进展提供进一步的洞察力，并为CC治疗提供一个有前途的靶点。
Cervical cancer (CC) is one of the most frequent gynecological malignancies in females worldwide. Aberrant expression of microRNA (miR)-206 was reported to play an important role in tumor progression. The aim of the present study was to evaluate the potential role of miR-206 and verify its influence on the function of glucose-6-phosphate dehydrogenase (G6PD) in CC. Western blot analysis and RT-PCR were employed to measure miR-206 and G6PD expression. Luciferase assays were performed to validate G6PD as miR-206 targets. CCK-8 assay was performed to examine the regulation of miR-206 and G6PD on CC proliferation. The result showed that miR-206 was downregulated, while G6PD was upregulated in high risk human papillomavirus (HR-HPV)(+) CC. miR-206 directly targeted the 3′-UTR of G6PD. miR-206 overexpressed or G6PD low-expressed suppressed cell proliferation. miR-206 low expressed or G6PD overexpressed predicted poor prognosis. In conclusion, miR-206 reduced cancer growth and suppresses the G6PD expression in CC. This newly identified miR-206 may provide further insight into tumor progression and offers a promising target for the CC therapy.
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