Virus pathotype and deep sequencing of the HA gene of a low pathogenicity H7N1 avian influenza virus causing mortality in Turkeys.
Virus pathotype and deep sequencing of the HA gene of a low pathogenicity H7N1 avian influenza virus causing mortality in Turkeys.
复制标题
DOI：
10.1371/journal.pone.0087076
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
McCauley JW
中科院分区：
文献类型：
--
作者：
Iqbal M;Reddy KB;Brookes SM;Essen SC;Brown IH;McCauley JW
H7亚型的低致病性禽流感（LPAI）病毒通常在家禽中引起轻度疾病。然而，LPAI病毒进化成高致病性禽流感（HPAI）病毒导致产生可引起严重疾病和死亡的病毒。这两种致病型的分类部分基于鸡的疾病体征和死亡，如在静脉内致病性试验中评估的，但LPAI病毒在火鸡中的作用尚不清楚。在对火鸡的LPAI病毒感染的调查期间，接种A/鸡/意大利/1279/99（H7 N1）的三周龄的鸡群显示出严重的疾病体征，并且在感染后七天内死亡或被安乐死。在被扑杀的禽类的许多内部组织和器官中检测到病毒。为了检查感染病毒在这些火鸡中进化为高致病性形式的可能性，通过采用桑格和下一代测序分析由拭子和组织样品提取物制备的多个cDNA扩增子，对血凝素（HA）基因切割位点进行序列分析。此外，还开发了一种检测高致病性禽流感病毒的RT-PCR方法。在用作接种物的病毒或从受感染禽类采集的拭子中均没有证据表明存在HPAI病毒。然而，个别气管或肝脏样本中携带的一小部分（<0.5%）病毒在HA切割位点确实含有HPAI病毒的典型分子特征。所有的签名序列是相同的，并在1999/2000年意大利流行病期间收集的高致病性禽流感病毒相似。我们假设在用A/鸡/意大利/1279/99感染后在组织样品中检测到HPAI病毒反映了在混合接种物中以非常低的水平存在的病毒的扩增，但是，引人注目的是，我们在通过深度测序分析的扩增DNA中没有观察到新的HPAI病毒特征。
Low pathogenicity avian influenza (LPAI) viruses of the H7 subtype generally cause mild disease in poultry. However the evolution of a LPAI virus into highly pathogenic avian influenza (HPAI) virus results in the generation of a virus that can cause severe disease and death. The classification of these two pathotypes is based, in part, on disease signs and death in chickens, as assessed in an intravenous pathogenicity test, but the effect of LPAI viruses in turkeys is less well understood. During an investigation of LPAI virus infection of turkeys, groups of three-week-old birds inoculated with A/chicken/Italy/1279/99 (H7N1) showed severe disease signs and died or were euthanised within seven days of infection. Virus was detected in many internal tissues and organs from culled birds. To examine the possible evolution of the infecting virus to a highly pathogenic form in these turkeys, sequence analysis of the haemagglutinin (HA) gene cleavage site was carried out by analysing multiple cDNA amplicons made from swabs and tissue sample extracts employing Sanger and Next Generation Sequencing. In addition, a RT-PCR assay to detect HPAI virus was developed. There was no evidence of the presence of HPAI virus in either the virus used as inoculum or from swabs taken from infected birds. However, a small proportion (<0.5%) of virus carried in individual tracheal or liver samples did contain a molecular signature typical of a HPAI virus at the HA cleavage site. All the signature sequences were identical and were similar to HPAI viruses collected during the Italian epizootic in 1999/2000. We assume that the detection of HPAI virus in tissue samples following infection with A/chicken/Italy/1279/99 reflected amplification of a virus present at very low levels within the mixed inoculum but, strikingly, we observed no new HPAI virus signatures in the amplified DNA analysed by deep-sequencing.
登录
查看更多内容
影响因子：
3.8
作者：
Garcia, M;Crawford, JM;Perdue, ML
通讯作者：
Perdue, ML
影响因子：
2.8
作者：
Capua, I;Mutinelli, F;Alexander, DJ
通讯作者：
Alexander, DJ
影响因子：
5
作者：
Dundon, William G.;Milani, Adelaide;Capua, Ilaria
通讯作者：
Capua, Ilaria
影响因子：
2.7
作者：
Banks, J;Speidel, ES;Alexander, DJ
通讯作者：
Alexander, DJ
DOI：
10.1098/rstb.2009.0088
发表时间：
2009-09-27
影响因子：
6.3
作者：
Iqbal, Munir;Xiao, Hiaxia;McCauley, John W.
通讯作者：
McCauley, John W.