The lamin B receptor‐associated protein p34 shares sequence homology and antigenic determinants with the splicing factor 2‐associated protein p32
The lamin B receptor‐associated protein p34 shares sequence homology and antigenic determinants with the splicing factor 2‐associated protein p32
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核纤层蛋白 B 受体相关蛋白 p34 与剪接因子 2 相关蛋白 p32 具有序列同源性和抗原决定簇
DOI：
10.1016/0014-5793(94)00479-x
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
S. Georgatos
中科院分区：
文献类型：
--
作者：
George Simos;S. Georgatos
层粘连蛋白B受体(P58)是一种内核膜蛋白，它与核层蛋白、核包膜蛋白和另外两种核蛋白形成体内复合体，其表观分子数分别为18,000(P18)和34,000(P34)。我们现在报告通过部分解离免疫亲和纯化的p58蛋白复合体来分离p34。对纯化的p34的N端氨基酸序列测定表明，该多肽与剪接因子2(SF2)相关蛋白p32同源。通过证明针对p32的N-末端和C-末端的抗体与纯化的p34发生交叉反应，可以进一步确定p34和p32之间的相关性。由于p58的氨基酸序列含有一个精氨酸/丝氨酸(RS)富集区，与SF2中发现的RS富集区相似，我们推测这些区域为p34提供了结合部位，该蛋白可能是核膜和核内剪接体亚结构之间的连接物。
The lamin B receptor (p58) is an inner nuclear membrane protein that forms an in vivo complex with the nuclear lamins, a nuclear envelope kinase, and two other nuclear proteins with apparentMrof 18,000 (p18) and 34,000 (p34). We now report the isolation of p34 by partial dissociation of the immunoaffinity-purified p58 protein complex. Determination of the N-terminal amino acid sequence of purified p34 shows that this polypeptide is homologous to p32, a splicing factor 2 (SF2)-associated protein. The relatedness between p34 and p32 can be further established by showing that antibodies raised against N- and C-terminal peptides of p32 cross-react with purified p34. As the amino acid sequence of p58 contains an arginine/serine (RS)-rich region similar to the RS-rich region found in SF 2, we speculate that these domains provide binding sites for p34 and that this protein may be a linker between the nuclear membrane and intranuclear spliceosomal substructures.
影响因子：
10.5
作者：
KRAINER, AR;CONWAY, GC;KOZAK, D
通讯作者：
KOZAK, D
核膜中的核纤层蛋白 B 受体。
DOI：
10.1073/pnas.85.22.8531
发表时间：
1988
影响因子：
11.1
作者：
Worman,HJ;Yuan,J;Blobel,G;Georgatos,SD
通讯作者：
Georgatos,SD