Assessing Anti-Adipogenic Effects of Mango Leaf Tea and Mangiferin within Cultured Adipocytes.
Assessing Anti-Adipogenic Effects of Mango Leaf Tea and Mangiferin within Cultured Adipocytes.
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DOI：
10.3390/diseases11020070
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发表时间：
2023-05-10
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影响因子：
--
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中科院分区：
文献类型：
--
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肥胖是由脂肪组织过剩引起的一种状况，是几种饮食相关疾病的危险因素。肥胖是一种全球性流行病，有效治疗也是一项挑战。然而，一种被推广的安全治疗肥胖的疗法是抗脂肪生成疗法。因此，鉴别出能安全用于临床的强效抗脂肪生物活性化合物可能会有效治疗人类肥胖。芒果叶具有潜在的药用价值，因为它含有许多生物活性化合物，可以促进人体健康。芒果苷（MGF）是芒果植物的主要成分，具有许多促进健康的品质。因此，本研究考察了MGF和芒果叶冲泡茶对体外培养脂肪细胞的影响。评估芒果叶茶（MLT）和MGF在3T3-L1细胞中的抗脂肪生成功效，并分析细胞活力、甘油三酯水平、脂联素分泌和葡萄糖摄取。此外，采用实时荧光定量PCR检测3T3-L1细胞内脂质代谢相关基因mRNA表达的变化。我们的研究结果显示，虽然MLT和MGF都增加了脂肪细胞的葡萄糖摄取，但只有MLT似乎抑制了脂肪生成，这是通过降低甘油三酯积累来确定的。我们还观察到，在MLT而非MGF处理的3T3-L1细胞中，分泌脂联素水平升高，ACC mRNA表达降低，FOXO1和ATGL基因表达升高。总之，这些结果表明，MLT可能具有独立于MGF含量的抗脂肪生成特性。
Obesity is a condition caused by surplus adipose tissue and is a risk factor for several diet-related diseases. Obesity is a global epidemic that has also been challenging to treat effectively. However, one promoted therapy to safely treat obesity is anti-adipogenic therapeutics. Therefore, identifying potent anti-adipogenic bioactive compounds that can safely be used clinically may effectively treat obesity in humans. Mango leaf has potential medicinal properties due to its many bioactive compounds that may enhance human health. Mangiferin (MGF) is a primary constituent in mango plants, with many health-promoting qualities. Therefore, this study investigated the effect of MGF, and tea brewed with mango leaves in cultured adipocytes. The anti-adipogenic efficacy of mango leaf tea (MLT) and MGF in 3T3-L1 cells were assessed, along with cell viability, triglyceride levels, adiponectin secretion, and glucose uptake analyzed. In addition, changes in the mRNA expression of genes involved in lipid metabolism within 3T3-L1 cells were determined using quantitative real-time PCR. Our results showed while both MLT and MGF increased glucose uptake in adipocytes, only MLT appeared to inhibit adipogenesis, as determined by decreased triglyceride accumulation. We also observed increased secretory adiponectin levels, reduced ACC mRNA expression, and increased FOXO1 and ATGL gene expression in 3T3-L1 cells treated with MLT but not MGF. Together, these results suggest that MLT may exhibit anti-adipogenic properties independent of MGF content.
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影响因子：
1.9
作者：
Esobi IC;Barksdale C;Heard-Tate C;Reigers Powell R;Bruce TF;Stamatikos A
通讯作者：
Stamatikos A
DOI：
10.1111/dom.12124
发表时间：
2014-02
期刊：
Diabetes, obesity & metabolism
影响因子：
--
作者：
Garg SK;Maurer H;Reed K;Selagamsetty R
通讯作者：
Selagamsetty R
影响因子：
5.3
作者：
Kim, Sun-Joong;Tang, Tianyi;Sul, Hei Sook
通讯作者：
Sul, Hei Sook
影响因子：
15.9
作者：
Denechaud, Pierre-Damien;Bossard, Pascale;Postic, Catherine
通讯作者：
Postic, Catherine
影响因子：
7.7
作者：
Kershaw, EE;Hamm, JK;Flier, JS
通讯作者：
Flier, JS