Chaihu Longgu Muli Decoction relieving temporal lobe epilepsy in rats by inhibiting TLR4 signaling pathway through miR-146a-3p and miR-146a-5p
Chaihu Longgu Muli Decoction relieving temporal lobe epilepsy in rats by inhibiting TLR4 signaling pathway through miR-146a-3p and miR-146a-5p
复制标题
柴胡龙骨牡蛎汤通过miR-146a-3p和miR-146a-5p抑制TLR4信号通路缓解大鼠颞叶癫痫
DOI：
10.1016/j.dcmed.2022.10.008
复制
发表时间：
2022-09
影响因子：
--
通讯作者：
PENG Qinghua
中科院分区：
文献类型：
--
作者：
MAO Yizhi;LI Liang;LUO Zhihong;HUANG Yahui;WU Huaying;YANG Ping;PENG Qinghua
ObjectiveTo explore the effect and mechanism of Chaihu Longgu Muli Decoction (柴胡龙骨牡蛎汤, CHLGMLD) in rats with temporal lobe epilepsy (TLE).MethodsA total of 80 Sprague-Dawley (SD) male rats were randomized into control (CON), model (MOD), carbamazepine (CBZ, 0.1 g/kg), CHLGMLD low dose (CHLGMLD-L, 12.5 g/kg), and high dose (CHLGMLD-H, 25 g/kg) groups, with 16 rats in each group. TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group. After the successful establishment of TLE models, all drugs were administered through gavage, and distilled water was given to rats in the CON and MOD groups for four weeks. The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-146a-3p and miR-146a-5p. The expression levels of toll-like receptor 4 (TLR4), interleukin-1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), TAK1-binding protein (TAB), nuclear factor-kappa B (NF-κB), and interleukin-1 beta (IL-1β) in hippocampus were tested by immunofluorescence assay. Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.ResultsCHLGMLD decreased the frequency (P< 0.05) and duration (P< 0.01) of seizures in rats. CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p (P< 0.05), and inhibited the expression levels of TLR4, IRAK1, TRAF6, TAB, NF-κB, and IL-1β(P< 0.01). The correlation analysis revealed that the expression levels of TLR4, IRAK1, TRAF6, TAB, NF-κB, and IL-1βwere positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR, respectively (P< 0.01).ConclusionCHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats, thus relieving seizures.
登录
查看更多内容
人脐带间充质干细胞来源的外泌体 miR-146a-5p 通过抑制缺血性中风后的 IRAK1/TRAF6 信号通路来减少小胶质细胞介导的神经炎症。
DOI：
10.18632/aging.202466
发表时间：
2021-01-21
期刊：
Aging
影响因子：
--
作者：
Zhang Z;Zou X;Zhang R;Xie Y;Feng Z;Li F;Han J;Sun H;Ouyang Q;Hua S;Lv B;Hua T;Liu Z;Cai Y;Zou Y;Tang Y;Jiang X
通讯作者：
Jiang X
影响因子：
3
作者：
Wong SQ;Jones A;Dodd S;Grimes D;Barclay JW;Marson AG;Cunliffe VT;Burgoyne RD;Sills GJ;Morgan A
通讯作者：
Morgan A
影响因子：
6.1
作者：
Yang P;Sun M;Li L;Shen Y
通讯作者：
Shen Y
影响因子：
3.4
作者：
Huilong Zhang;Y. Qu;Aihua Wang
通讯作者：
Huilong Zhang;Y. Qu;Aihua Wang
影响因子：
4.3
作者：
Fanyou Ning;Shaobo Zhu;Hui Gao;Yu Deng
通讯作者：
Fanyou Ning;Shaobo Zhu;Hui Gao;Yu Deng