Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates.
Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates.
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两光子荧光光谱和4-二甲基氨基酰胺肽肽和蛋白结合物的成像。
DOI：
10.1021/jp407321g
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发表时间：
2013-12-19
期刊：
影响因子：
--
通讯作者：
Goodson T 3rd
中科院分区：
文献类型：
--
作者：
McLean AM;Socher E;Varnavski O;Clark TB;Imperiali B;Goodson T 3rd
我们报告详细的双光子荧光过程的溶剂化显色荧光团4-DMN的共轭物的重要钙调素（CaM）和相关的钙调素结合肽M13的光物理研究。已观察到与钙结合相关的强双光子荧光增强。据发现，双光子吸收截面是强烈依赖于周围的4-DMN荧光团在钙调素共轭物的局部环境，提供荧光团连接的网站之间的灵敏度。利用时间分辨测量，4-DMN在各种环境（溶剂）条件下的排放动力学进行了分析。此外，各向异性测量显示，4-DMN-S38 C-CaM系统在钙结合的钙调蛋白中具有限制性旋转。为了建立细胞成像的实用性，还在细胞中用4-DMN修饰的M13肽进行了双光子荧光显微镜研究。总之，这些研究提供了强有力的证据表明，4-DMN是一个有用的探针在双光子成像，细胞实验的有利性能。
We report detailed photophysical studies on the two-photon fluorescence processes of the solvatochromic fluorophore 4-DMN as a conjugate of the important calmodulin (CaM) and the associated CaM-binding peptide M13. Strong two-photon fluorescence enhancement has been observed which is associated with calcium binding. It is found that the two-photon absorption cross-section is strongly dependent on the local environment surrounding the 4-DMN fluorophore in the CaM conjugates, providing sensitivity between sites of fluorophore attachment. Utilizing time-resolved measurements, the emission dynamics of 4-DMN under various environmental (solvent) conditions are analyzed. In addition, anisotropy measurements reveal that the 4-DMN-S38C-CaM system has restricted rotation in the calcium-bound calmodulin. To establish the utility for cellular imaging, two-photon fluorescence microscopy studies were also carried out with the 4-DMN-modified M13 peptide in cells. Together, these studies provide strong evidence that 4-DMN is a useful probe in two-photon imaging, with advantageous properties for cellular experiments.
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DOI：
10.1016/j.jphotochem.2009.06.007
发表时间：
2009-08-15
影响因子：
4.3
作者：
Nag, Amit;Goswami, Debabrata
通讯作者：
Goswami, Debabrata
影响因子：
4
作者：
Black, DJ;Tran, QK;Persechini, A
通讯作者：
Persechini, A
影响因子：
15
作者：
Bhaskar, Ajit;Guda, Ramakrishna;Goodson, Theodore, III
通讯作者：
Goodson, Theodore, III
影响因子：
2.9
作者：
Guo, Meng;Varnavski, Oleg;Goodson, Theodore, III
通讯作者：
Goodson, Theodore, III
影响因子：
14.8
作者：
Lemke, Edward A.;Schultz, Carsten
通讯作者：
Schultz, Carsten