Evaluating optical coherence tomography for surgical margin assessment of canine mammary tumours.
Evaluating optical coherence tomography for surgical margin assessment of canine mammary tumours.
复制标题
DOI：
10.1111/vco.12632
复制
发表时间：
2021-12
影响因子：
2.1
通讯作者：
Boppart SA
中科院分区：
文献类型：
--
作者：
Fabelo C;Selmic LE;Huang PC;Samuelson JP;Reagan JK;Kalamaras A;Wavreille V;Monroy GL;Marjanovic M;Boppart SA
光学相干断层扫描（OCT）利用近红外光波在微观尺度上产生实时、高分辨率的图像，类似于低功率组织病理学。先前的研究已经证明OCT用于人类乳腺癌的实时手术切缘评估。使用OCT对犬乳腺肿瘤（CMT）可以术中观察手术边缘的残余肿瘤。本研究的目的是评估OCT成像对CMT术后肿瘤切除不全的检测。我们假设OCT图像与CMT手术切除边缘组织的组织病理学图像具有相似的特征，并且OCT检测CMT手术切除不完全的灵敏度很高。从19只接受CMT手术切除的客户犬中获得30个手术标本。术缘脂肪组织、皮肤组织、乳腺组织和乳腺肿瘤的光学相干断层成像的外观和特征各不相同。手术边缘正常和异常组织的OCT图像被用来建立一个用于观察者评估的OCT图像数据集。离体图像的灵敏度和特异性分别为83.3%和82.0%（观察者1）和70.0%和67.9%（观察者2）。对活体图像的敏感性和特异性分别为70.0%和89.3%（观察者1），76.7%和67.9%（观察者2）。这些结果表明OCT可能用于CMT的手术切缘评估，以优化手术干预和临床结果。对观察员进行更好的培训和经验可以提高敏感性和特异性。
Optical coherence tomography (OCT) uses near-infrared light waves to generate real-time, high-resolution images on the microscopic scale similar to low power histopathology. Previous studies have demonstrated the use of OCT for real-time surgical margin assessment for human breast cancer. The use of OCT for canine mammary tumors (CMT) could allow intra-operative visualization of residual tumor at surgical margins. The purpose of this study was to assess OCT imaging for the detection of incomplete tumor resection following CMT surgery. We hypothesized that OCT images would have comparable features to histopathological images of tissues at the surgical margins of CMT resections along with a high sensitivity of OCT detection of incomplete surgical excision of CMT. Thirty surgical specimens were obtained from nineteen client-owned dogs undergoing surgical resection of CMT. Optical coherence tomography image appearance and characteristics of adipose tissue, skin, mammary tissue and mammary tumor at surgical margins were distinct and different. The OCT images of normal and abnormal tissues at surgical margins were utilized to develop a dataset of OCT images for observer evaluation. The sensitivity and specificity for ex vivo images were 83.3% and 82.0% (observer 1) and 70.0% and 67.9% (observer 2). The sensitivity and specificity for in vivo images were 70.0% and 89.3% (observer 1), and 76.7% and 67.9% (observer 2). These results indicate a potential use of OCT for surgical margin assessment for CMT to optimize surgical intervention and clinical outcomes. Improved training and experience of observers may improve sensitivity and specificity.
登录
查看更多内容
影响因子：
2.1
作者：
Selmic LE;Samuelson J;Reagan JK;Mesa KJ;Driskell E;Li J;Marjanovic M;Boppart SA
通讯作者：
Boppart SA
影响因子：
2.4
作者：
Goldschmidt, M.;Pena, L.;Zappulli, V.
通讯作者：
Zappulli, V.
影响因子：
6.2
作者：
Neuschatz, AC;DiPetrillo, T;Wazer, DE
通讯作者：
Wazer, DE
DOI：
10.2460/javma.240.10.1181
发表时间：
2012-05-15
影响因子：
1.9
作者：
Scarpa, Filippo;Sabattini, Silvia;Bettini, Giuliano
通讯作者：
Bettini, Giuliano
影响因子：
2.4
作者：
Mesa KJ;Selmic LE;Pande P;Monroy GL;Reagan J;Samuelson J;Driskell E;Li J;Marjanovic M;Chaney EJ;Boppart SA
通讯作者：
Boppart SA