Acetylcholine α7 nicotinic and dopamine D2 receptors are targeted to many of the same postsynaptic dendrites and astrocytes in the rodent prefrontal cortex.
Acetylcholine α7 nicotinic and dopamine D2 receptors are targeted to many of the same postsynaptic dendrites and astrocytes in the rodent prefrontal cortex.
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DOI：
10.1002/syn.20977
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发表时间：
2011-12
期刊：
影响因子：
2.3
通讯作者：
Pickel, Virginia M.
中科院分区：
文献类型：
--
作者：
Duffy, Aine M.;Fitzgerald, Megan L.;Chan, June;Robinson, Danielle C.;Milner, Teresa A.;Mackie, Kenneth;Pickel, Virginia M.
α -7烟碱乙酰胆碱受体（α7nAChR）和多巴胺D2受体（D2R）都与注意力过程和认知有关，部分通过前额叶皮层（PFC）介导。我们检测了α7nAChR和D2R或囊状乙酰胆碱转运蛋白（VAChT）在啮齿动物PFC中的双电镜免疫标记，以评估会聚功能激活位点。α7nAChR和/或D2R的免疫反应性（ir）在相同以及单独的神经元和胶质谱中可见。至少一半的双标记谱是体细胞和树突，而大多数标记轴突末端只表达D2R-ir。D2R标记的末端无突触特化或形成抑制性或兴奋性突触，具有体树突结构，其中部分表达α7nAChR和/或D2R。星形细胞胶质过程包括大部分非体树突α7nAChR或α7nAChR和d2r标记的谱。含有α7nAChR-ir的胶质突通常位于vacht标记的末梢附近，也显示突触周围和血管周围的关联。我们得出结论，在啮齿类动物PFC中，α7nACh和D2R的激活可以双重调节(1)在相同或独立但突触连接的神经元中，D2R主要分布在突触前，突触后树突反应；(2)星形细胞信号可能对突触传递和功能性充血至关重要。
The alpha-7 nicotinic acetylcholine receptor (α7nAChR) and the dopamine D2 receptor (D2R) are both implicated in attentional processes and cognition, mediated in part through the prefrontal cortex (PFC). We examined the dual electron microscopic immunolabeling of α7nAChR and either D2R or the vesicular acetylcholine transporter (VAChT) in rodent PFC to assess convergent functional activation sites. Immunoreactivity (ir) for α7nAChR and/or D2R was seen in the same as well as separate neuronal and glial profiles. At least half of the dually labeled profiles were somata and dendrites, while most labeled axon terminals expressed only D2R-ir. The D2R-labeled terminals were without synaptic specializations or formed inhibitory or excitatory-type synapses with somatodendritic profiles, some of which expressed the α7nAChR and/or D2R. Astrocytic glial processes comprised the majority of nonsomatodendritic α7nAChR or α7nAChR and D2R-labeled profiles. Glial processes containing α7nAChR-ir were frequently located near VAChT-labeled terminals and also showed perisynaptic and perivascular associations. We conclude that in rodent PFC α7nACh and D2R activation can dually modulate (1) postsynaptic dendritic responses within the same or separate but synaptically linked neurons in which the D2R has the predominately presynaptic distribution, and (2) astrocytic signaling that may be crucial for synaptic transmission and functional hyperemia.
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