Genomic characteristics and comparative genomics analysis of Salmonella enterica subsp. enterica serovar Thompson isolated from an outbreak in South Korea.
Genomic characteristics and comparative genomics analysis of Salmonella enterica subsp. enterica serovar Thompson isolated from an outbreak in South Korea.
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DOI：
10.1038/s41598-022-22168-2
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发表时间：
2022-11-29
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
沙门氏菌感染是一个重要的公共卫生问题。2018年，美国多个州爆发了肠道沙门氏菌亚种。据报道，韩国学校受污染的巧克力蛋糕导致肠血清型汤普森感染。在这项研究中，我们对从巧克力蛋糕、蛋清、蜜饯和与疫情有关的炊具中分离出的37株S. Thompson菌株进行了测序。此外，我们分析了61株S. Thompson菌株的基因组序列（37株与巧克力蛋糕相关的爆发株，4株来自韩国的爆发株，20株来自国家生物技术信息中心），通过比较基因组学和系统发育分析来评估爆发相关菌株的基因组特征。结果显示，同分类聚集性将菌株分为A、I亚聚集群（含2018年在韩国的菌株）和B、II亚聚集群（含2014 - 2015年在韩国的菌株）。从韩国分离的S. Thompson与公开的菌株进行了准确的区分。与其他S. Thompson基因组不同，巧克力蛋糕爆发相关菌株的染色体上整合了三种沙门氏菌噬菌体（SEN8， vB SosS Oslo和SI7）。比较基因组学揭示了几个基因负责巧克力蛋糕爆发相关菌株的特定基因组特征，以及三种可能有助于其他S. Thompson菌株致病性的噬菌体。
Salmonella infections represent an important public health problem. In 2018, a multistate outbreak of S. enterica subsp. enterica serovar Thompson infection associated with contaminated chocolate cakes in schools was reported in South Korea. In this study, we sequenced the 37 S. Thompson strains isolated from chocolate cakes, egg whites, preserves, and cookware associated with the outbreak. In addition, we analyze the genomic sequences of 61 S. Thompson strains (37 chocolate cake-related outbreak strains, 4 strains isolated from outbreaks in South Korea and 20 strains available in the National Center for Biotechnology Information) to assess the genomic characteristics of outbreak-related strains by comparative genomics and phylogenetic analysis. The results showed that identically classified clusters divided strains into two clusters, sub-clusters A & I (with strains from 2018 in South Korea) and sub-clusters B & II (with strains from 2014 to 2015 in South Korea). S. Thompson isolated from South Korea were accurately distinguished from publicly-available strains. Unlike other S. Thompson genomes, those of chocolate cake outbreak-related strains had three Salmonella phages (SEN8, vB SosS Oslo, and SI7) integrated into their chromosome. Comparative genomics revealed several genes responsible for the specific genomic features of chocolate cake outbreak-related strains and three bacteriophages that may contribute to the pathogenicity of other S. Thompson strains.
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影响因子：
5.2
作者：
Pightling AW;Pettengill JB;Luo Y;Baugher JD;Rand H;Strain E
通讯作者：
Strain E
影响因子：
6.4
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Campbell, JV;Mohle-Boetani, J;Werner, SB
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Werner, SB
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Mulvey, Matthew A.
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Gillespie, S. H.
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4.2
作者：
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通讯作者：
Werner, SB