Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics.
Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics.
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DOI：
10.1186/1465-9921-6-110
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发表时间：
2005-09-22
影响因子：
5.8
通讯作者：
Venge P
中科院分区：
文献类型：
--
作者：
Amin K;Janson C;Sevéus L;Miyazaki K;Virtanen I;Venge P
层粘连蛋白是一组主要负责细胞锚定到基底膜的蛋白质。我们假设支气管粘膜层粘连蛋白链产生的改变可能解释哮喘中上皮细胞脱落的现象。目的是表征过敏性和非过敏性哮喘患者SEBM和上皮中层粘连蛋白链的存在。对11例过敏性哮喘患者和9例非过敏性哮喘患者以及7例对照患者进行支气管活检，并对层粘连蛋白（ln）链α 1- α 5、β 1- β 2和γ 1- γ 2进行抗体染色。Lns-2、lns -5和lns -10是SEBM的主要层状蛋白。两个哮喘组的ln-10层变厚，而只有变应性哮喘组的ln- 2和ln -5层厚度增加。ln γ - 2链仅在ln 5中发现，在与上皮损伤相关的上皮细胞和过敏性哮喘患者的柱状上皮中只表达。过敏性哮喘患者ln-5链的不协调产生可能与这些患者上皮细胞锚定不良有关。
Laminins are a group of proteins largely responsible for the anchorage of cells to basement membranes. We hypothesized that altered Laminin chain production in the bronchial mucosa might explain the phenomenon of epithelial cell shedding in asthma. The aim was to characterize the presence of Laminin chains in the SEBM and epithelium in allergic and non-allergic asthmatics. Biopsies were taken from the bronchi of 11 patients with allergic and 9 patients with non-allergic asthma and from 7 controls and stained with antibodies against the Laminin (ln) chains alpha1-alpha5, beta1-beta2 and gamma1-gamma2. Lns-2,-5 and -10 were the main Laminins of SEBM. The layer of ln-10 was thicker in the two asthmatic groups while an increased thickness of lns-2 and -5 was only seen in allergic asthmatics. The ln gamma2-chain, which is only found in ln 5, was exclusively expressed in epithelial cells in association with epithelial injury and in the columnar epithelium of allergic asthmatics. The uncoordinated production of chains of ln-5 in allergic asthma could have a bearing on the poor epithelial cell anchorage in these patients.
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DOI：
10.1091/mbc.1.10.731
发表时间：
1990-09-01
期刊：
CELL REGULATION
影响因子：
--
作者：
ENGVALL, E;EARWICKER, D;SANES, JR
通讯作者：
SANES, JR
影响因子：
14.2
作者：
Kelly, EAB;Busse, WW;Jarjour, NN
通讯作者：
Jarjour, NN
影响因子：
4.8
作者：
Tiger, CF;Champliaud, MF;Gullberg, D
通讯作者：
Gullberg, D
影响因子：
3.7
作者：
Virtanen, I;Gullberg, D;Ekblom, P
通讯作者：
Ekblom, P
影响因子：
12.4
作者：
Lúdvíksdottir, D;Janson, C;Valtysdóttir, S
通讯作者：
Valtysdóttir, S