Conditional gene targeting: dissecting the cellular mechanisms of retinal degenerations.
Conditional gene targeting: dissecting the cellular mechanisms of retinal degenerations.
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DOI：
10.1155/2011/806783
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发表时间：
2011
影响因子：
1.9
通讯作者：
Le YZ
中科院分区：
文献类型：
--
作者：
Le YZ
视网膜神经元的退化和存活通常受到胚胎发育所需的相同营养因子的调控，并且通常在多种细胞类型中表达。因此，条件基因打靶方法是研究视网膜变性中广泛表达和发育调节基因的细胞特异性功能的必要手段。本文将重点讨论基于CRE/LOX的条件性基因打靶方法在视网膜变性机制研究中的应用。除了基本的实验设计外，本文还讨论了影响条件性基因打靶研究结果的各种因素，现有技术的局限性，各种视网膜细胞的Cre驱动系的可用性，以及与Cre表达小鼠的生成相关的问题。最后，本文综述了基于Cre/lox的基因打靶方法在视网膜变性机制研究中的应用现状，包括杆状感光细胞在光氧化应激下的存活和蛋白质在感光细胞中的运输。
Retinal neuron degeneration and survival are often regulated by the same trophic factors that are required for embryonic development and are usually expressed in multiple cell-types. Therefore, the conditional gene targeting approach is necessary to investigate the cell-specific function of widely expressed and developmentally regulated genes in retinal degeneration. The discussion in this review will be focused on the use of Cre/lox-based conditional gene targeting approach in mechanistic studies for retinal degeneration. In addition to the basic experimental designs, this article addresses various factors influencing the outcomes of conditional gene targeting studies, limitations of current technologies, availability of Cre-drive lines for various retinal cells, and issues related to the generation of Cre-expressing mice. Finally, this review will update the current status on the use of Cre/lox-based gene targeting approach in mechanistic studies for retinal degeneration, which includes rod photoreceptor survival under photo-oxidative stress and protein trafficking in photoreceptors.
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影响因子：
3.3
作者：
Ivanova, E.;Hwang, G. -S.;Pan, Z. -H.
通讯作者：
Pan, Z. -H.
DOI：
10.1073/pnas.93.20.10887
发表时间：
1996-10-01
影响因子：
11.1
作者：
Feil, R;Brocard, J;Chambon, P
通讯作者：
Chambon, P
影响因子：
14.9
作者：
Gagneten, S;Le, YZ;Sauer, B
通讯作者：
Sauer, B
影响因子：
3.5
作者：
Churchill, Amanda J.;Carter, James G.;Atan, Denize
通讯作者：
Atan, Denize
影响因子：
64.8
作者：
Guo, F;Gopaul, DN;VanDuyne, GD
通讯作者：
VanDuyne, GD