Characterization of transgenic mouse lines expressing Cre recombinase in the retina.
Characterization of transgenic mouse lines expressing Cre recombinase in the retina.
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DOI：
10.1016/j.neuroscience.2009.10.021
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发表时间：
2010-01-13
期刊：
影响因子：
3.3
通讯作者：
Pan, Z. -H.
中科院分区：
文献类型：
--
作者：
Ivanova, E.;Hwang, G. -S.;Pan, Z. -H.
哺乳动物的视网膜由五大类神经元细胞和神经胶质细胞组成，它包含50多种细胞类型。在视网膜特定细胞类型中操纵基因表达的能力对于理解视网膜功能和疾病的分子机制非常重要。Cre/loxP重组系统已被证明是一个强大的工具，允许基因缺失，过表达和异位表达在体内以细胞和组织特异性的方式。该工具的关键是Cre小鼠系具有Cre重组酶的细胞或组织型特异性表达。迄今为止，已经产生了大量的Cre转基因小鼠系，目的是将Cre重组酶表达到中枢神经系统的特定神经元和胶质细胞群中；然而，关于Cre重组酶系在视网膜中的表达模式的信息在很大程度上缺乏。在本研究中，我们检测并表征了Cre重组酶在15个Cre转基因小鼠视网膜中的表达模式。在这些细胞系中，大多数细胞系都观察到Cre诱导的重组或Cre重组酶的表达。特别是，我们发现了几种Cre系，其中Cre诱导的重组被发现只针对或主要针对单一类型或类别的视网膜细胞，包括双层视网膜神经节细胞、星爆无突细胞、杆双极细胞和m<s:1> ller胶质细胞。在其他细胞系中，在几种视网膜细胞类型中发现了cre诱导的重组。这些Cre系为视网膜研究提供了宝贵的资源。
The mammalian retina consists of five major classes of neuronal cells, as well as glial cells, and it contains more than 50 cell types. The ability to manipulate gene expression in specific cell type(s) in the retina is important for understanding the molecular mechanisms of retinal function and diseases. The Cre/loxP recombination system has been shown to be a powerful tool, allowing gene deletion, over-expression, and ectopic expression in vivo in a cell- and tissue-specific fashion. The key to this tool is the availability of Cre mouse lines with cell- or tissue-type specific expression of Cre recombinase. To date, a large number of Cre-transgenic mouse lines have been generated to target Cre recombinase expression to specific neuronal and glial cell populations in the CNS; however, information about the expression patterns of Cre recombinase lines in the retina is largely lacking. In this study, we examined and characterized the expression patterns of Cre recombinase in the retinas of 15 Cre-transgenic mouse lines. Significant Cre-induced recombination or expression of Cre recombinase was observed in the majority of these lines. In particular, we found several Cre lines in which the Cre-induced recombination was found to target exclusively or predominantly a single type or class of retinal cells, including bistratified retinal ganglion cells, starburst amacrine cells, rod bipolar cells, and Müller glial cells. In other lines, the Cre-induced recombination was found in several retinal cell types. These Cre lines provide a valuable resource for retinal research.
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