Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes.
Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes.
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DOI：
10.1038/s41396-022-01188-w
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发表时间：
2022-05
期刊：
影响因子：
11
通讯作者：
Zhu, Yong-Guan
中科院分区：
文献类型：
--
作者：
Zheng, Xiaoxuan;Jahn, Martin T.;Sun, Mingming;Friman, Ville-Petri;Balcazar, Jose Luis;Wang, Jinfeng;Shi, Yu;Gong, Xin;Hu, Feng;Zhu, Yong-Guan
病毒通过编码与能量获取、胁迫耐受和外源物质降解相关的多种辅助代谢基因（AMG），对局部和全球的生物地球化学循环产生重要影响，并帮助细菌在不同环境中生存。本文研究了中国有机氯农药污染土壤中细菌（dsDNA）病毒编码的AMG是否富集，以及病毒AMG中是否含有与有机氯农药生物降解相关的基因。使用宏基因组学，我们发现OCP污染的土壤显示出较低的细菌，但较高的病毒多样性，这些病毒具有较高的相对丰度与农药降解和代谢相关的AMG。此外，随着农药污染程度的加重，AMG的多样性和相对丰度沿着显著增加，并在病毒宏基因组中鉴定出多个生物降解基因。进行功能测定以实验证明病毒编码的L-2-卤酸脱卤酶基因（L-DEX）负责降解L-2-卤酸农药前体，从而改善细菌在亚抑制农药浓度下的生长。总之，这些结果表明，病毒编码的AMG与细菌代谢和生物降解有关，在农药污染的土壤中更丰富和多样。此外，我们的研究结果强调了病毒编码的辅助基因对压力环境中细菌生态的重要性，为使用病毒对受污染土壤进行生物修复提供了一种新的途径。
Viruses significantly influence local and global biogeochemical cycles and help bacteria to survive in different environments by encoding various auxiliary metabolic genes (AMGs) associated with energy acquisition, stress tolerance and degradation of xenobiotics. Here we studied whether bacterial (dsDNA) virus encoded AMGs are enriched in organochlorine pesticide (OCP) contaminated soil in China and if viral AMGs include genes linked to OCP biodegradation. Using metagenomics, we found that OCP-contaminated soils displayed a lower bacterial, but higher diversity of viruses that harbored a higher relative abundance of AMGs linked to pesticide degradation and metabolism. Furthermore, the diversity and relative abundance of AMGs significantly increased along with the severity of pesticide contamination, and several biodegradation genes were identified bioinformatically in viral metagenomes. Functional assays were conducted to experimentally demonstrate that virus-encoded L-2-haloacid dehalogenase gene (L-DEX) is responsible for the degradation of L-2-haloacid pesticide precursors, improving bacterial growth at sub-inhibitory pesticide concentrations. Taken together, these results demonstrate that virus-encoded AMGs are linked to bacterial metabolism and biodegradation, being more abundant and diverse in soils contaminated with pesticides. Moreover, our findings highlight the importance of virus-encoded accessory genes for bacterial ecology in stressful environments, providing a novel avenue for using viruses in the bioremediation of contaminated soils.
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影响因子：
15.5
作者：
Adriaenssens EM;Kramer R;Van Goethem MW;Makhalanyane TP;Hogg I;Cowan DA
通讯作者：
Cowan DA
影响因子：
11
作者：
Hurwitz, Bonnie L.;Brum, Jennifer R.;Sullivan, Matthew B.
通讯作者：
Sullivan, Matthew B.
影响因子：
64.8
作者：
Al-Shayeb, Basem;Sachdeva, Rohan;Banfield, Jillian F.
通讯作者：
Banfield, Jillian F.
影响因子：
56.9
作者：
Brum, Jennifer R.;Ignacio-Espinoza, J. Cesar;Sullivan, Matthew B.
通讯作者：
Sullivan, Matthew B.
DOI：
10.1073/pnas.1525139113
发表时间：
2016-03-01
影响因子：
11.1
作者：
Brum, Jennifer R.;Ignacio-Espinoza, J. Cesar;Sullivan, Matthew B.
通讯作者：
Sullivan, Matthew B.