A phylogenomic analysis of the Actinomycetales mce operons.
A phylogenomic analysis of the Actinomycetales mce operons.
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DOI：
10.1186/1471-2164-8-60
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发表时间：
2007-02-26
期刊：
影响因子：
4.4
通讯作者：
Riley LW
中科院分区：
文献类型：
--
作者：
Casali N;Riley LW
结核分枝杆菌的基因组含有一组称为mce操纵子的基因的四个拷贝。尽管广泛的研究已经证明了这些操纵子对感染结果的重要性，但它们的生理功能仍然不清楚。扩大完整微生物基因组序列的数据库，促进比较基因组学的方法，可以提供有价值的洞察未知蛋白质的作用。分枝结核病mce基因座各包括两个yrbE和六个mce基因，它们分别与ABC转运蛋白通透酶和底物结合蛋白具有同源性。具有相同结构的操纵子被确定在所有分枝杆菌物种检查，以及在其他五个放线菌属。一些放线菌目mce操纵子包括mkl基因，其编码类似于ABC摄取转运蛋白的ATP酶。Mkl直系同源物的系统发育谱与Mce和YrbE蛋白的系统发育谱完全匹配。通过对YrbE同源物的拓扑结构和基序分析，我们确定了倒数第二个细胞质环内的一个区域，该区域可以作为与推定的同源Mkl ATP酶相互作用的位点。与M.在大多数放线菌目操纵子下游的保守染色体位置检测到结核MCE操纵子。操纵子含有连接的mkl，yrbE和mce基因，类似于ABC进口商的经典组织，被发现是常见的革兰氏阴性菌，似乎与细胞表面的性质的变化。提出的证据表明，放线菌目物种的MCE操纵子和革兰氏阴性菌中的相关操纵子编码ABC摄取转运蛋白的一个亚家族，其可能在重塑细胞包膜中起作用。
The genome of Mycobacterium tuberculosis harbors four copies of a cluster of genes termed mce operons. Despite extensive research that has demonstrated the importance of these operons on infection outcome, their physiological function remains obscure. Expanding databases of complete microbial genome sequences facilitate a comparative genomic approach that can provide valuable insight into the role of uncharacterized proteins. The M. tuberculosis mce loci each include two yrbE and six mce genes, which have homology to ABC transporter permeases and substrate-binding proteins, respectively. Operons with an identical structure were identified in all Mycobacterium species examined, as well as in five other Actinomycetales genera. Some of the Actinomycetales mce operons include an mkl gene, which encodes an ATPase resembling those of ABC uptake transporters. The phylogenetic profile of Mkl orthologs exactly matched that of the Mce and YrbE proteins. Through topology and motif analyses of YrbE homologs, we identified a region within the penultimate cytoplasmic loop that may serve as the site of interaction with the putative cognate Mkl ATPase. Homologs of the exported proteins encoded adjacent to the M. tuberculosis mce operons were detected in a conserved chromosomal location downstream of the majority of Actinomycetales operons. Operons containing linked mkl, yrbE and mce genes, resembling the classic organization of an ABC importer, were found to be common in Gram-negative bacteria and appear to be associated with changes in properties of the cell surface. Evidence presented suggests that the mce operons of Actinomycetales species and related operons in Gram-negative bacteria encode a subfamily of ABC uptake transporters with a possible role in remodeling the cell envelope.
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Riley, LW