Site-directed mutagenesis of coenzyme-independent carotenoid oxygenase CSO2 to enhance the enzymatic synthesis of vanillin
Site-directed mutagenesis of coenzyme-independent carotenoid oxygenase CSO2 to enhance the enzymatic synthesis of vanillin
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不依赖辅酶的类胡萝卜素加氧酶 CSO2 的定点诱变增强香草醛的酶促合成
DOI：
10.1007/s00253-020-10433-1
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发表时间：
2020-03
影响因子：
5
通讯作者：
Fengjiao Xin
中科院分区：
文献类型：
--
作者：
Xueyan Yao;Yuemeng Lv;Huilei Yu;Hao Cao;Luyao Wang;Boting Wen;Tianyi Gu;Fengzhong Wang;Lichao Sun;Fengjiao Xin
Vanillin is a popular flavoring compound and an important food additive. Owing to the consumer preference for inexpensive natural aroma flavors, vanillin production through a biotechnological pathway has become of great interest and commercial value in recent years. In this study, an enzymatic synthetic system for vanillin using a coenzyme-independent decarboxylase (FDC) and oxygenase (CSO2) cascade was reconstituted and optimized. This system produces a slightly higher production yield (40.20%) than the largest yield reported for immobilized FDC and CSO2 (35.00%) with ferulic acid as a substrate. It was previously reported that the low catalytic activity and thermal instability of CSO2 restrict the overall productivity of vanillin. In present study, site-directed mutagenesis was applied to rate-limiting oxygenase CSO2 to generate positive mutants. The production yields of mutants A49P (58.44%) and Q390A (65.29%) were 1.45- and 1.62-fold that of CSO2 wild type, respectively. The potential mechanism for enhanced vanillin production using A49P involved increased thermostability and catalytic efficiency, while that using Q390A was probably associated with a better thermostable performance and increased catalytic efficiency resulting from a larger entrance channel.
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影响因子：
3.4
作者：
Di Gioia, Diana;Sciubba, Luigi;Fava, Fabio
通讯作者：
Fava, Fabio
影响因子：
4.1
作者：
N. A. Zamzuri;S. Abd-Aziz
通讯作者：
N. A. Zamzuri;S. Abd-Aziz
DOI：
10.1111/febs.13232
发表时间：
2015-04
期刊：
The FEBS journal
影响因子：
--
作者：
Boone CD;Rasi V;Tu C;McKenna R
通讯作者：
McKenna R
影响因子：
3
作者：
Lesage-Meessen, L;Lomascolo, A;Asther, M
通讯作者：
Asther, M
影响因子：
56.9
作者：
Schwartz, SH;Tan, BC;McCarty, DR
通讯作者：
McCarty, DR