Serum exosomal microRNA-146a as a novel diagnostic biomarker for acute coronary syndrome.
Serum exosomal microRNA-146a as a novel diagnostic biomarker for acute coronary syndrome.
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血清外泌体 microRNA-146a 作为急性冠状动脉综合征的新型诊断生物标志物
DOI：
10.21037/jtd-21-609
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发表时间：
2021-05
影响因子：
2.5
通讯作者：
Meng ZH
中科院分区：
文献类型：
--
作者：
Li LJ;Gu YJ;Wang LQ;Wan W;Wang HW;Yang XN;Ma LL;Yang LH;Meng ZH
循环microRNA（miRNAs）已成为心血管疾病的潜在生物标志物。然而，很少有研究关注外泌体miRNA在急性冠状动脉综合征（ACS）中的作用。本研究的目的是探讨血清外泌体microRNA-146 a（exo-miR-146 a）是否可作为ACS的新型诊断生物标志物，并探讨其与炎症反应的关系。ACS患者63例，对照组25例。分离血清外泌体，然后通过透射电子显微镜（TEM）、蛋白质印迹和纳米颗粒跟踪分析（NTA）鉴定。采用实时定量聚合酶链反应（RT-qPCR）检测血清中exo-miR-146 a的表达水平，采用酶联免疫吸附试验（ELISA）检测血清中白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的表达水平。采用斯皮尔曼相关分析评价血清exo-miR-146 a的潜在相关因素，并采用受试者工作特征（ROC）曲线分析通过曲线下面积（AUC）预测ACS的准确性。从血清中分离的外来体具有典型的杯状形状，直径为50-150 nm，并表达CD 9、CD 63、CD 81和HSP 70。ACS患者血清中exo-miR-146 a、IL-1β、IL-6和TNF-α的表达水平较对照组显著升高，斯皮尔曼相关分析显示exo-miR-146 a与IL-1β、IL-6和TNF-α的表达水平呈显著正相关。ROC曲线分析显示exo-miR-146 a可以区分ACS患者和正常对照。血清exo-miR-146 a可能作为ACS患者的一种新的诊断生物标志物，并且与炎症反应相关。
Circulating microRNAs (miRNAs) have emerged as potential biomarkers for cardiovascular diseases. However, few studies have focused on the role of exosomal miRNAs in acute coronary syndrome (ACS). The purpose of this study was to explore weather serum exosomal microRNA-146a (exo-miR-146a) could be used as a novel diagnostic biomarker for ACS and to investigate its relationship with inflammatory response. A total of 63 ACS patients and 25 patients with normal coronary arteries (Control) were enrolled respectively. The serum exosomes were isolated and then identified by transmission electron microscopy (TEM), western blot, and nanoparticle tracking analysis (NTA). The expression levels of exo-miR-146a in serum were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and the expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in serum were assessed by enzyme-linked immunosorbent assay (ELISA). Spearman’s correlation analysis was used to appraise the potential factors related to serum exo-miR-146a and receiver operating characteristic (ROC) curve analysis was applied for predicting the accuracy of ACS via the area under curve (AUC). Exosomes isolated from serum were of typical cup-like shape, with 50-150 nm diameter, and expressed CD9, CD63, CD81, and HSP70. The expression levels of serum exo-miR-146a, IL-1β, IL-6, and TNF-α were significantly increased in ACS patients compared with the control group, Spearman′s correlation analysis indicated that exo-miR-146a expression was markedly positively correlated with IL-1β, IL-6, and TNF-α. The ROC curve analyses revealed that exo-miR-146a could distinguish ACS patients from their normal controls. The serum exo-miR-146a may be used as a novel diagnostic biomarker for ACS patients, and it is also associated with inflammatory response.
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